Evidence map›Paper›PMID 33458736›Full record

ArticleGenetics and molecular biology2021

Genomic sequencing of different sequevars of Ralstonia solanacearum belonging to the Moko ecotype.

Ana Karolina Leite Pais, Jessica Rodrigues da Silva, Leandro Victor Silva Dos Santos, Greecy Mirian Rodrigues Albuquerque, Antonio Roberto Gomes de Farias, Wilson José Silva Junior, Valdir de Queiroz Balbino, Adriano Márcio Freire Silva, Marco Aurélio Siqueira da Gama, Elineide Barbosa de Souza

Abstract read
In one paragraph

Article in Genetics and molecular biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ana Karolina Leite PaisUniversidade Federal Rural de Pernambuco (UFRPE), Departamento de Agronomia, Recife, PE, Brazil.ORCID http://orcid.org/0000-0003-4058-6678
Jessica Rodrigues da SilvaInstituto Federal de Educação, Ciência e Tecnologia do Piauí (IFPI), Piauí, PI, Brazil.
Leandro Victor Silva Dos SantosUniversidade Federal Rural de Pernambuco (UFRPE), Departamento de Agronomia, Recife, PE, Brazil.
Greecy Mirian Rodrigues AlbuquerqueUniversidade Federal Rural de Pernambuco (UFRPE), Departamento de Agronomia, Recife, PE, Brazil.
Antonio Roberto Gomes de FariasUniversidade Federal Rural de Pernambuco (UFRPE), Departamento de Agronomia, Recife, PE, Brazil.ORCID http://orcid.org/0000-0003-4768-1547
Wilson José Silva JuniorUniversidade Federal de Pernambuco (UFPE), Departamento de Genética, Recife, PE, Brazil.
Valdir de Queiroz BalbinoUniversidade Federal de Pernambuco (UFPE), Departamento de Genética, Recife, PE, Brazil.
Adriano Márcio Freire SilvaUniversidade Federal de Alagoas (UFAL), Departamento de Agronomia, Maceió, AL, Brazil.
Marco Aurélio Siqueira da GamaUniversidade Federal Rural de Pernambuco (UFRPE), Departamento de Agronomia, Recife, PE, Brazil.
Elineide Barbosa de SouzaUniversidade Federal Rural de Pernambuco (UFRPE), Departamento de Biologia, Recife, PE, Brazil.ORCID http://orcid.org/0000-0003-1281-3490

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Banana vascular wilt or Moko is a disease caused by Ralstonia solanacearum. This study aimed to sequence, assemble, annotate, and compare the genomes of R. solanacearum Moko ecotypes of different sequevar strains from Brazil. Average nucleotide identity analyses demonstrated a high correlation (> 96%) between the genome sequences of strains CCRMRs277 (sequevar IIA-24), CCRMRs287 (IIB-4), CCRMRs304 (IIA-24), and CCRMRsB7 (IIB-25), which were grouped into phylotypes IIA and IIB. The number of coding sequences present in chromosomes and megaplasmids varied from 3,070 to 3,521 and 1,669 to 1,750, respectively. Pangenome analysis identified 3,378 clusters in the chromosomes, of which 2,604 were shared by all four analyzed genomes and 2,580 were single copies. In megaplasmids, 1,834 clusters were identified, of which 1,005 were shared by all four genomes and 992 were identified as single copies. Strains CCRMRsB7 and CCRMRs287 differed from the others by having unique clusters in both their chromosomes and megaplasmids, and CCRMRsB7 possessed the largest genome among all Moko ecotype strains sequenced to date. Therefore, the genomic information obtained in this study provides a theoretical basis for the identification, characterization, and phylogenetic analysis of R. solanacearum Moko ecotypes.

Identifiers

PMID33458736
PMCPMC7917543

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.