Evidence map›Paper›PMID 33458620›Full record

ArticleiScience2021

Vitamin D sufficiency enhances differentiation of patient-derived prostate epithelial organoids.

Tara McCray, Julian V Pacheco, Candice C Loitz, Jason Garcia, Bethany Baumann, Michael J Schlicht, Klara Valyi-Nagy, Michael R Abern, Larisa Nonn

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 1 synthesis or guideline pooled it.

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  20. Harnessing the Utility ofFrontiers in oncology · 2022
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Tara McCrayDepartment of Pathology, University of Illinois at Chicago, 840 S Wood Street, Chicago, IL 60612, USA.
Julian V PachecoDepartment of Pathology, University of Illinois at Chicago, 840 S Wood Street, Chicago, IL 60612, USA.
Candice C LoitzDepartment of Pathology, University of Illinois at Chicago, 840 S Wood Street, Chicago, IL 60612, USA.
Jason GarciaDepartment of Pathology, University of Illinois at Chicago, 840 S Wood Street, Chicago, IL 60612, USA.
Bethany BaumannDepartment of Pathology, University of Illinois at Chicago, 840 S Wood Street, Chicago, IL 60612, USA.
Michael J SchlichtDepartment of Pathology, University of Illinois at Chicago, 840 S Wood Street, Chicago, IL 60612, USA.
Klara Valyi-NagyDepartment of Pathology, University of Illinois at Chicago, 840 S Wood Street, Chicago, IL 60612, USA.
Michael R AbernUniversity of Illinois Cancer Center, Chicago, IL 60612, USA.
Larisa NonnDepartment of Pathology, University of Illinois at Chicago, 840 S Wood Street, Chicago, IL 60612, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vitamin D is an essential steroid hormone that regulates systemic calcium homeostasis and cell fate decisions. The prostate gland is hormonally regulated, requiring steroids for proliferation and differentiation of secretory luminal cells. Vitamin D deficiency is associated with an increased risk of lethal prostate cancer, which exhibits a dedifferentiated pathology, linking vitamin D sufficiency to epithelial differentiation. To determine vitamin D regulation of prostatic epithelial differentiation, patient-derived benign prostate epithelial organoids were grown in vitamin D-deficient or -sufficient conditions. Organoids were assessed by phenotype and single-cell RNA sequencing. Mechanistic validation demonstrated that vitamin D sufficiency promoted organoid growth and accelerated differentiation by inhibiting canonical Wnt activity and suppressing Wnt family member DKK3. Wnt and DKK3 were also reduced by vitamin D in prostate tissue explants by spatial transcriptomics. Wnt dysregulation is a known contributor to aggressive prostate cancer, thus findings further link vitamin D deficiency to lethal disease.

Indexed as

Cell BiologyDevelopmental BiologyTranscriptomics

Identifiers

PMID33458620
PMCPMC7797919

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.