ArticleACS omega2021
Adaptive Optical Two-Photon Microscopy for Surface-Profiled Living Biological Specimens.
Article in ACS omega, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Model-based aberration corrected microscopy inside a glass tube.Journal of microscopy · 2025Article
- Large-scale cranial window for in vivo mouse brain imaging utilizing fluoropolymer nanosheet and light-curable resin.Communications biology · 2024Article
- Spatial frequency-based correction of the spherical aberration in living brain imaging.Microscopy (Oxford, England) · 2024Article
- Improving two-photon excitation microscopy for sharper and faster biological imaging.Biophysics and physicobiology · 2023Article
- Fluorescence radial fluctuation enables two-photon super-resolution microscopy.Frontiers in cellular neuroscience · 2023Article
- Complex-Amplitude-Modulation Vectorial Excitation Beam for High-Resolution Observation of Deep Regions in Two-Photon Microscopy.Frontiers in neuroscience · 2022Article
- Protocol for constructing an extensive cranial window utilizing a PEO-CYTOP nanosheet forSTAR protocols · 2021Article
- 3DeeCellTracker, a deep learning-based pipeline for segmenting and tracking cells in 3D time lapse images.eLife · 2021Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed adaptive optical (AO) two-photon excitation microscopy by introducing a spatial light modulator (SLM) in a commercially available microscopy system. For correcting optical aberrations caused by refractive index (RI) interfaces at a specimen's surface, spatial phase distributions of the incident excitation laser light were calculated using 3D coordination of the RI interface with a 3D ray-tracing method. Based on the calculation, we applied a 2D phase-shift distribution to a SLM and achieved the proper point spread function. AO two-photon microscopy improved the fluorescence image contrast in optical phantom mimicking biological specimens. Furthermore, it enhanced the fluorescence intensity from tubulin-labeling dyes in living multicellular tumor spheroids and allowed successful visualization of dendritic spines in the cortical layer V of living mouse brains in the secondary motor region with a curved surface. The AO approach is useful for observing dynamic physiological activities in deep regions of various living biological specimens with curved surfaces.
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Registered trials
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