ArticleBiochemistry and biophysics reports2021
Fourier-Transform InfraRed (FT-IR) spectroscopy to show alterations in molecular composition of EV subpopulations from melanoma cell lines in different malignancy.
Article in Biochemistry and biophysics reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- TopicalPharmaceutics · 2026Article
- Expanding the Analytical Toolbox for Extracellular Vesicle Biochemical Profiling: A Multiplatform Spectroscopic and Chromatographic Strategy.Analytical chemistry · 2026Article
- Exosome-based layered double hydroxide nanohybrids from adipose-derived stem cells enhance dermal wound healing.Scientific reports · 2026Article
- Targeted delivery of doxorubicin using RSV F-protein modified breast cancer-derived exosomes in breast cancer-bearing mice.BioImpacts : BI · 2026Article
- Plasma-induced modification of fenugreek galactomannan thickener film via dielectric barrier discharge for textile printing.Scientific reports · 2025Article
- Diagnostic System for Early In Situ Melanoma Detection Using Acoustic Microscopy and Infrared Spectroscopic Mapping Imaging.Cancers · 2025Article
- Microfluidic Devices for Manufacture of Therapeutic Extracellular Vesicles: Advances and Opportunities.Journal of extracellular vesicles · 2025Review
- Biogenesis and functional implications of extracellular vesicles in cancer metastasis.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2025Review
- Article
- Extracellular Vesicles from Different Mesenchymal Stem Cell Types Exhibit Distinctive Surface Protein Profiling and Molecular Characteristics: A Comparative Analysis.International journal of molecular sciences · 2025Article
- Multimodal Nanobiophysical Profiling of Melanoma-Derived Small Extracellular Vesicles Reveals Glycan Signatures Associated with Tumor Progression.Nanotechnology, science and applications · 2025Article
- Size matters: Biomolecular compositions of small and large extracellular vesicles in the urine of glioblastoma patients.Journal of extracellular biology · 2024Article
- Spectroscopic insight into breast cancer: profiling small extracellular vesicles lipids via infrared spectroscopy for diagnostic precision.Scientific reports · 2024Article
- Aminated Rapeseed Husks (Molecules (Basel, Switzerland) · 2024Article
- miRNA Signature of Urine Extracellular Vesicles Shows the Involvement of Inflammatory and Apoptotic Processes in Diabetic Chronic Kidney Disease.Pharmaceutical research · 2023Article
- Multiparametric Biosensors for Characterizing Extracellular Vesicle Subpopulations.ACS pharmacology & translational science · 2023Article
- The Convergence of FTIR and EVs: Emergence Strategy for Non-Invasive Cancer Markers Discovery.Diagnostics (Basel, Switzerland) · 2022Review
- Antenna-enhanced mid-infrared detection of extracellular vesicles derived from human cancer cell cultures.Journal of nanobiotechnology · 2022Article
- Exosome Structures Supported by Machine Learning Can Be Used as a Promising Diagnostic Tool.Materials (Basel, Switzerland) · 2022Article
- Extracellular vesicles derived from microgreens of Raphanus sativus L. var. caudatus Alef contain bioactive macromolecules and inhibit HCT116 cells proliferation.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMelanoma cells release extracellular vesicles (EVs) subpopulations which differ in size, phenotype and molecular content. Melanoma derived EVs play a role in the development and progression of cancer by delivering surface receptors and bioactive (proteins, lipids, nucleic acids) or signaling molecules to target cells.
methodsWe applied Fourier Transform Infrared Spectroscopy (FTIR) to compare infrared spectra of absorption for different subpopulations of EVs originating from normal human melanocytes, primary cutaneous melanoma (WM115) and metastatic cutaneous melanoma (WM266-4).
resultsFTIR results showed that exosome and ectosome populations differ in content of protein and lipid components. We obtained higher lipid to protein ratio for ectosomes in comparison with exosomes what confirms that exosomes are very densely packed with protein cargo. We identified the lowest value of saturated fatty acids/unsaturated fatty acids parameter in the metastatic WM266-4 cell line and ectosomes derived from WM266-4 cell line in comparison with normal melanocytes and the primary WM115 cell line. We identified the alterations in the content of secondary structures of proteins present in EV subpopulations originating from melanocytes and melanoma cells in different malignancy.
conclusionsObtained results revealed differences in the molecular composition of melanoma derived EVs subtypes, including protein secondary structure, and showed progressive structural changes during cancer development.
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