ArticleAdvanced biomedical research2020
Detection of Dermatophytes from Dermatophytosis-Suspected Cases in Iran, Evaluation of Polymerase Chain Reaction-Sequencing Method.
Article in Advanced biomedical research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Comparative Evaluation of Conventional and Molecular Methods for the Diagnosis of Onychomycosis.Cureus · 2026Article
- Comparative evaluation of conventional and nanopore approaches in onychomycosis diagnosis.Diagnostic microbiology and infectious disease · 2026Article
- Comparison of in vitro activities of newer triazoles and classic antifungal agents against dermatophyte species isolated from Iranian University Hospitals: a multi-central study.Annals of clinical microbiology and antimicrobials · 2023Article
- Epidemiology of Dermatophytes Isolated from Clinical Samples in a Hospital in Eastern Saudi Arabia: A 20-Year Survey.Journal of epidemiology and global health · 2021Article
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Authors and funding
5 authors.
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Abstract
backgroundDermatophytosis is mostly caused by dermatophytes species, and the diagnosis of disease is very important for early treatment. The aim of this study was to identify the commonly dermatophytes species isolated directly from the clinical samples, using the polymerase chain reaction (PCR) and evaluate both conventional and molecular methods. MATERIALS AND
methodsThis study was performed on 115 clinical samples. Dermatophyte isolates were initially identified by conventional method and confirmed by the sequencing molecular method. In this study, the molecular technique is implemented directly on clinical samples. Statistical analysis of the information was performed by the SPSS software, and the results were statistically analyzed.
resultsOur findings demonstrated that the most abundant dermatophyte species by PCR-sequencing were
conclusionFor medical laboratories, routine procedures are still preferred because of their lower cost, and the results are almost the same as the molecular methods. The sensitivity and specificity values for PCR under our laboratory condition were 60% and 87%, respectively. This study shows that molecular results performed better in nails than other samples, by culture results.
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