Evidence map›Paper›PMID 33455403›Full record

ArticleACS biomaterials science & engineering2020

Growth and Spatial Control of Murine Neural Stem Cells on Reflectin Films.

Rylan Kautz, Long Phan, Janahan Arulmoli, Atrouli Chatterjee, Justin P Kerr, Mahan Naeim, James Long, Alex Allevato, Jessica E Leal-Cruz, LeAnn Le and 4 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS biomaterials science & engineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Structure, self-assembly, and properties of a truncated reflectin variant.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 1 institution in 1 country.

Rylan KautzDepartment of Chemical Engineering and Materials Science, University of California, Irvine, 916 Engineering Tower, Irvine, California 92697, United States.
Long PhanDepartment of Chemical Engineering and Materials Science, University of California, Irvine, 916 Engineering Tower, Irvine, California 92697, United States.
Janahan ArulmoliDepartment of Biomedical Engineering, University of California, Irvine, 3120 Natural Sciences II, Irvine, California 92697, United States.
Atrouli ChatterjeeDepartment of Chemical Engineering and Materials Science, University of California, Irvine, 916 Engineering Tower, Irvine, California 92697, United States.
Justin P KerrDepartment of Mechanical and Aerospace Engineering, University of California, Irvine, 4200 Engineering Gateway Building, Irvine, California 92697, United States.
Mahan NaeimDepartment of Biomedical Engineering, University of California, Irvine, 3120 Natural Sciences II, Irvine, California 92697, United States.
James LongDepartment of Chemical Engineering and Materials Science, University of California, Irvine, 916 Engineering Tower, Irvine, California 92697, United States.
Alex AllevatoDepartment of Chemical Engineering and Materials Science, University of California, Irvine, 916 Engineering Tower, Irvine, California 92697, United States.
Jessica E Leal-CruzDepartment of Chemical Engineering and Materials Science, University of California, Irvine, 916 Engineering Tower, Irvine, California 92697, United States.
LeAnn LeDepartment of Chemical Engineering and Materials Science, University of California, Irvine, 916 Engineering Tower, Irvine, California 92697, United States.
Parsa DerakhshanDepartment of Chemical Engineering and Materials Science, University of California, Irvine, 916 Engineering Tower, Irvine, California 92697, United States.
Francesco TombolaDepartment of Physiology and Biophysics, University of California, Irvine, 825 Health Sciences Road, Irvine, California 92697, United States.ORCID 0000-0002-9315-0352
Lisa A FlanaganDepartment of Biomedical Engineering, University of California, Irvine, 3120 Natural Sciences II, Irvine, California 92697, United States.
Alon A GorodetskyDepartment of Chemical Engineering and Materials Science, University of California, Irvine, 916 Engineering Tower, Irvine, California 92697, United States.ORCID 0000-0002-3811-552X
University of California, Irvine · US

Funding

Univ.of Calif., Irvine Cancer Center Support GrantP30CA062203 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Melanie Funes · 1994 to 2026
$57.9M
Mechanisms of Permeation and Gating of Voltage-Sensing DomainsR01GM098973 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI TOMBOLA, FRANCESCO · 2011 to 2025
$4.5M
Training Program in Stem Cell Translational Medicine for Neurological DisordersT32NS082174 · NINDS · UNIVERSITY OF CALIFORNIA-IRVINE · PI PETER John DONOVAN, Leslie Michels Thompson · 2013 to 2026
$3.3M
PacBio RS Single Molecule, Real-Time (SMRT) DNA SequencerS10OD010794 · OD · UNIVERSITY OF CALIFORNIA-IRVINE · PI SANDMEYER, SUZANNE · 2012 to 2012
$600k
High-Throughput DNA SequencerS10OD021718 · OD · UNIVERSITY OF CALIFORNIA-IRVINE · PI SANDMEYER, SUZANNE · 2016 to 2016
$600k
High Throughput DNA SequencerS10RR025496 · NCRR · UNIVERSITY OF CALIFORNIA-IRVINE · PI SANDMEYER, SUZANNE · 2009 to 2009
$500k
NCI NIH HHS P30 CA062203NCRR NIH HHS S10 RR025496NIGMS NIH HHS R01 GM098973NIH HHS S10 OD010794NIH HHS S10 OD021718NINDS NIH HHS T32 NS082174
6 · The paper itself

Abstract

Stem cells have attracted significant attention due to their regenerative capabilities and their potential for the treatment of disease. Consequently, significant research effort has focused on the development of protein- and polypeptide-based materials as stem cell substrates and scaffolds. Here, we explore the ability of reflectin, a cephalopod structural protein, to support the growth of murine neural stem/progenitor cells (mNSPCs). We observe that the binding, growth, and differentiation of mNSPCs on reflectin films is comparable to that on more established protein-based materials. Moreover, we find that heparin selectively inhibits the adhesion of mNSPCs on reflectin, affording spatial control of cell growth and leading to a >30-fold change in cell density on patterned substrates. The described findings highlight the potential utility of reflectin as a stem cell culture material.

Indexed as

CephalopodaNeural Stem CellsAnimalsCell DifferentiationCell ProliferationMiceProteinsProteinsbiomaterialscell adhesioncephalopodsheparinpatterning

Identifiers

PMID33455403
PMCPMC7833438
OpenAlexW3000890184

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.