Evidence map›Paper›PMID 33455201›Full record

ArticleACS biomaterials science & engineering2021

Single-Cell Quantification of the Transition Temperature of Intracellular Elastin-like Polypeptides.

David R Tyrpak, Yaocun Li, Siqi Lei, Hugo Avila, John Andrew MacKay

Open access · greenAbstract read
In one paragraph

Article in ACS biomaterials science & engineering, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

David R TyrpakDepartment of Pharmacology and Pharmaceutical Sciences, School of Pharmacy of the University of Southern California, 1985 Zonal Avenue, Los Angeles, California 90089, United States.
Yaocun LiDepartment of Pharmacology and Pharmaceutical Sciences, School of Pharmacy of the University of Southern California, 1985 Zonal Avenue, Los Angeles, California 90089, United States.
Siqi LeiDepartment of Pharmacology and Pharmaceutical Sciences, School of Pharmacy of the University of Southern California, 1985 Zonal Avenue, Los Angeles, California 90089, United States.
Hugo AvilaDepartment of Pharmacology and Pharmaceutical Sciences, School of Pharmacy of the University of Southern California, 1985 Zonal Avenue, Los Angeles, California 90089, United States.
John Andrew MacKayDepartment of Pharmacology and Pharmaceutical Sciences, School of Pharmacy of the University of Southern California, 1985 Zonal Avenue, Los Angeles, California 90089, United States.ORCID 0000-0002-3626-1654
University of Southern California · US

Funding

USC/NORRIS COMPREHENSIVE CANCER CENTER (CORE) SUPPORTP30CA014089 · NCI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Fumito Ito · 1985 to 2026
$181.4M
USC RESEARCH CENTER FOR LIVER DISEASES: PILOT PROGRAMSP30DK048522 · NIDDK · UNIVERSITY OF SOUTHERN CALIFORNIA · PI KAPLOWITZ, NEIL · 1995 to 2020
$21.8M
Ophthalmic Therapeutics Engineering CoreP30EY029220 · NEI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Mahnaz Shahidi · 2018 to 2026
$6.5M
Protein-polymer nanomedicine for Sjogren's SyndromeR01EY026635 · NEI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Sarah F Hamm-Alvarez, John Andrew MacKay · 2017 to 2026
$4.6M
Intracellular switching using genetically engineered protein microdomainsR01GM114839 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI MACKAY, JOHN ANDREW · 2015 to 2019
$1.9M
Novel temperature-responsive fusions of clathrin and caveolin to explore TGF-beta mediated fibrosisF31DK118881 · NIDDK · UNIVERSITY OF SOUTHERN CALIFORNIA · PI TYRPAK, DAVID RYAN · 2018 to 2019
$77k
NCI NIH HHS P30 CA014089NEI NIH HHS P30 EY029220NEI NIH HHS R01 EY026635NIDDK NIH HHS F31 DK118881NIDDK NIH HHS P30 DK048522NIGMS NIH HHS R01 GM114839
6 · The paper itself

Abstract

Elastin-like polypeptides (ELPs) are modular, stimuli-responsive materials that self-assemble into protein-rich microdomains in response to heating. By cloning ELPs to effector proteins, expressed intracellular fusions can even modulate cellular pathways. A critical step in engineering these fusions is to determine and control their intracellular phase transition temperature (

Indexed as

ElastinPeptidesPhase TransitionTemperatureTransition TemperatureElastinPeptidesgenetically engineered protein microdomainsGEPMquantitative fluorescence microscopytransition temperature

Identifiers

PMID33455201
PMCPMC8375696
OpenAlexW3124947800

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.