Evidence map›Paper›PMID 33454906›Full record

ArticleMolecular biology reports2021

Selection of reference genes for quantitative real-time PCR normalization in European quail tissues.

Fabiana Cristina Belchior de Sousa, Carlos Souza do Nascimento, Maíse Dos Santos Macário, Renan Dos Santos Araújo, Leandro Teixeira Barbosa, Geraldo Fábio Viana Bayão, Katiene Régia Silva Sousa

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Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Veterinary world · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Fabiana Cristina Belchior de SousaDepartment of Animal Science, Universidade Federal do Piauí, Campus Profª Cinobelina Elvas, Bom Jesus, PI, 64900-000, Brazil.
Carlos Souza do NascimentoDepartment of Animal Science, Universidade Federal de Sergipe, São Cristóvão, SE, 49100-000, Brazil.
Maíse Dos Santos MacárioDepartment of Animal Science, Universidade Federal de Sergipe, São Cristóvão, SE, 49100-000, Brazil.
Renan Dos Santos AraújoDepartment of Entomology, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil. renandosantosaraujo@gmail.com.ORCID http://orcid.org/0000-0002-8868-2012
Leandro Teixeira BarbosaDepartment of Animal Science, Universidade Federal de Sergipe, São Cristóvão, SE, 49100-000, Brazil.
Geraldo Fábio Viana BayãoDepartment of Animal Science, Instituto Federal de Educação, Ciência e Tecnologia do Maranhão, São Luís, MA, 65095-460, Brazil.
Katiene Régia Silva SousaDepartment of Oceanography and Limnology, Universidade Federal do Maranhão, São Luís, MA, 65080-805, Brazil.

Funding

Fundação CAPES Finance code 001
6 · The paper itself

Abstract

Coturniculture has been standing out as an industrial poultry activity in several countries around the world because of the several adaptive advantages of quails. Research that considers the analysis of gene expression can enhance this activity. This study aimed to analyze the stability of reference genes (RGs) in different tissues of quails (both males and females) for the recommendation of use in gene expression studies by the quantitative reverse transcription-polymerase chain reaction (RT-qPCR). The expression stability of ten RGs (ACTA1, ACTB, B2M, GAPDH, HMBS, SDHA, HPRT1, MRPS27, MRPS30, and RPL5) was analyzed in four tissues (breast muscle, abdominal fat, liver, and intestine), and assessed using the statistical tools geNorm, NormFinder, comparative ΔC

Indexed as

Reference StandardsAnimalsBreastFemaleGene Expression ProfilingIntestinal MucosaLiverMaleQuailReal-Time Polymerase Chain ReactionAbdominal fatBreast muscleEndogenous geneExpression stabilityIntestineLiver

Identifiers

PMID33454906

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.