ArticleMethods in enzymology2021
Cell-free reconstitution of multi-condensate assemblies.
Article in Methods in enzymology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Phase separation of PGL-3 driven by structured domains that oligomerize and interact with RGG motifs.EMBO reports · 2026Article
- The CCT chaperonin and actin modulate the ER and RNA-binding protein condensation during oogenesis and maintain translational repression of maternal mRNA and oocyte quality.Molecular biology of the cell · 2024Article
- What's past is prologue: FRAP keeps delivering 50 years later.Biophysical journal · 2023Review
- The Dynamic Regulation of mRNA Translation and Ribosome Biogenesis During Germ Cell Development and Reproductive Aging.Frontiers in cell and developmental biology · 2021Review
Corrections and comments
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Authors and funding
2 authors.
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Abstract
Biomolecular condensates (BCs) are intracellular condensates that form by phase separation of proteins and RNA from the nucleoplasm or cytoplasm. BCs often form complex assemblies where compositionally distinct condensates wet each other without mixing. In this chapter, we describe methods to reconstitute multi-condensate assemblies from purified components. We include protocols to express, purify, label, and analyze the dynamics of proteins and RNAs that drive multi-condensate assembly. Analysis of the condensation and wetting behaviors of condensates in cell-free reconstituted systems can be used to define the molecular interactions that regulate BCs in cells.
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