ArticleNature protocols2021
Mapping mechanical properties of biological materials via an add-on Brillouin module to confocal microscopes.
Article in Nature protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
57 citing papers in PubMed, 96 citations in OpenAlex.
- Acoustic cell patterning reveals geometry- and substrate-dependent vasculogenesis and human embryo model development.Nature communications · 2026Article
- Dual Optical Elastography Techniques Reveal the Regulation of Corneal Stiffness by Collagen XIV.Journal of biophotonics · 2026Article
- Article
- Wavelet time-frequency analysis enhances noise-resilient quantification of corneal wave velocity and natural frequency in optical coherence elastography.Biomedical optics express · 2026Article
- AGP and EXO-LIKE genes promote brassinosteroid-dependent anisotropic growth.The New phytologist · 2026Article
- Mechanical Stiffening Promotes Growth, Invasion, and Mitogen-Activated Protein Kinase Kinase (MEK) Inhibitor Resistance in 3D Plexiform Neurofibroma Cultures.bioRxiv : the preprint server for biology · 2026Article
- The mechanical properties ofScience advances · 2026Article
- Stabilized real-time Brillouin microscopy reveals fractal organization of protein condensates in living cells.Nature communications · 2026Article
- High-performance Brillouin spectroscopy using VIPA-etalon cascades.Journal of biomedical optics · 2026Article
- Plasmodesmata wall biomechanics: challenges and opportunities.Journal of experimental botany · 2026Review
- A framework for spontaneous Brillouin noise: unveiling fundamental limits in Brillouin metrology.Light, science & applications · 2026Article
- Macrophages Mediate Mesoscale Brain Mechanical Homeostasis.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Cell wall-derived mechanical signals control cell growth and division during root development.Science advances · 2025Article
- Brillouin microscopy in cancer research: a review.Journal of biomedical optics · 2025Review
- Clinical multimodal Brillouin microscopy-optical coherence elastography system for lens biomechanics.Journal of biomedical optics · 2025Article
- Cellular mechanical properties in response to environmental viscosity imaged by Brillouin Microscopy.Communications biology · 2025Article
- Mechanical contribution of germ cell specification inScience advances · 2025Article
- Understanding and measuring mechanical signals in the tumor stroma.FEBS open bio · 2025Review
- Imaging the mechanical properties of a developing embryonic tendon using Brillouin microscopy.Acta biomaterialia · 2025Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Several techniques have been developed over the past few decades to assess the mechanical properties of biological samples, which has fueled a rapid growth in the fields of biophysics, bioengineering, and mechanobiology. In this context, Brillouin optical spectroscopy has long been known as an intriguing modality for noncontact material characterization. However, limited by speed and sample damage, it had not translated into a viable imaging modality for biomedically relevant materials. Recently, based on a novel spectroscopy strategy that substantially improves the speed of Brillouin measurement, confocal Brillouin microscopy has emerged as a unique complementary tool to traditional methods as it allows noncontact, nonperturbative, label-free measurements of material mechanical properties. The feasibility and potential of this innovative technique at both the cell and tissue level have been extensively demonstrated over the past decade. As Brillouin technology is rapidly recognized, a standard approach for building and operating Brillouin microscopes is required to facilitate the widespread adoption of this technology. In this protocol, we aim to establish a robust approach for instrumentation, and data acquisition and analysis. By carefully following this protocol, we expect that a Brillouin instrument can be built in 5-9 days by a person with basic optics knowledge and alignment experience; the data acquisition as well as postprocessing can be accomplished within 2-8 h.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.