ArticleScientific reports2021
Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers, 2 of them syntheses that pooled it.
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Who cites it
61 citing papers in PubMed, 2 syntheses or guidelines pooled it, 103 citations in OpenAlex.
- The Effect of Probiotic Lactobacillus Strains on Porphyromonas gingivalis, the Etiological Factor of Periodontal Diseases-A Systematic Review.Probiotics and antimicrobial proteins · 2026Pooled it
- Postbiotics and Dental Caries: A Systematic Review.Clinical and experimental dental research · 2025Pooled it
- Intratumoral microbiota in tumorigenesis: A double-edged sword.Chinese medical journal · 2026Review
- Decoding the Applications of Microbial Metabolites: Insights into the Antibacterial Potential of Lactobacillus acidophilus Postbiotics.Current microbiology · 2026Article
- Infection control and anti-apoptotic effect of Lactobacillus paracasei derivatives on fibroblasts through modulation of BCL2 transcription.Archives of microbiology · 2026Article
- Culture filtrates of lactic acid bacteria promote the growth and enhance the resistance of tomato seedlings to Ralstonia solanacearum.BMC plant biology · 2026Article
- Inhibition of biofilm formation by Limosilactobacillus fermentum supernatant against Porphyromonas gingivalis and Fusobacterium nucleatum: an in-vitro study.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Engineered Lactobacillus reuteri for scavenging reactive oxygen species and modulating oral microflora in periodontitis therapy.International journal of oral science · 2026Article
- Effects of Oral Probiotics onDentistry journal · 2026Review
- Antimicrobial and Antibiofilm Activity of aBiomolecules · 2026Article
- Postbiotics from lactic acid bacteria as promising alternatives against urinary tract pathogens.Frontiers in microbiology · 2026Article
- Probiotic intervention modulates volatile sulfur compound-associated pathways and oral microbiome balance in ligature-induced periodontitis rats.Journal of oral microbiology · 2026Article
- Cell-Free but potent: Antimicrobial potential of Lactobacillus supernatants against multidrug-resistant pathogens.PloS one · 2026Article
- Source-dependent antimicrobial activity of plant-derived lactic acid bacteria against periodontal pathogens.Bioscience of microbiota, food and health · 2026Article
- Comparative inhibition of oral pathogens, biofilm formation, and inflammation-related responses by postbiotics fromJournal of oral microbiology · 2026Article
- Antibacterial and anti-biofilm effects of postbiotic mediator derived fromJournal of oral microbiology · 2026Article
- Lyophilized Cell-Free Supernatants ofLife (Basel, Switzerland) · 2025Article
- Article
- Fusobacterium nucleatum: a transboundary pathogen in host-microbiota networks.Gut pathogens · 2025Review
- Fusobacterium nucleatum and non-coding RNAs: orchestrating oncogenic pathways in colorectal cancer.Gut pathogens · 2025Review
1 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactobacillus reuteri AN417 is a newly characterized probiotic strain. The activity of AN417 against oral pathogenic bacteria is unknown. We investigated the antibacterial activity of cell-free L. reuteri AN417 culture supernatant (LRS) against three oral pathogens: Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mutans. P. gingivalis and F. nucleatum have been implicated in periodontal disease, whereas S. mutans causes dental caries. Exposing these oral pathogenic bacteria to LRS significantly reduced their growth rates, intracellular ATP levels, cell viability, and time-to-kill. The minimal inhibitory volume of LRS was 10% (v/v) against P. gingivalis, 20% (v/v) for F. nucleatum, and 30% (v/v) for S. mutans. LRS significantly reduced the integrity of biofilms and significantly suppressed the expression of various genes involved in P. gingivalis biofilm formation. The L. reuteri AN417 genome lacked genes encoding reuterin, reuteran, and reutericyclin, which are major antibacterial compounds produced in L. reuteri strains. LRS treated with lipase and α-amylase displayed decreased antibacterial activity against oral pathogens. These data suggest that the antibacterial substances in LRS are carbohydrates and/or fatty acid metabolites. Our results demonstrate that LRS has antimicrobial activity against dental pathogenic bacteria, highlighting its potential utility for the prevention and treatment of P. gingivalis periodontal disease.
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