ArticleNucleic acids research2021
The ATR-WEE1 kinase module inhibits the MAC complex to regulate replication stress response.
Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Replication Stress: A Review of Novel Targets to Enhance Radiosensitivity-From Bench to Clinic.Frontiers in oncology · 2022Pooled it
- Plant-specific and conserved mechanisms of the polymerase-associated factor 1 complex in replication stress responses.Nature plants · 2026Article
- LUMINIDEPENDENS orchestrates global transcriptional repression inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- The polymerase-associated factor 1 complex modulates the growth-defense tradeoff inScience advances · 2025Article
- Post-translational modifications of SOG1 enable dynamic control of plant DNA damage response.Science advances · 2025Article
- AtCHE1, the Arabidopsis homolog of mammalian AATF/Che-1 protein, is involved in safeguarding genome stability.Communications biology · 2025Article
- Regulation of mNature plants · 2025Article
- Integrative multi-omics analysis of metabolic dysregulation induced by occupational benzene exposure in mice.The Science of the total environment · 2025Article
- Pleiotropic regulatory locus 1 maintains actin cytoskeleton integrity and cellular homeostasis to enable Arabidopsis root growth.iScience · 2024Article
- Arabidopsis Histone Variant H2A.X Functions in the DNA Damage-Coupling Abscisic Acid Signaling Pathway.International journal of molecular sciences · 2024Article
- Mechanistic insights into DNA damage recognition and checkpoint control in plants.Nature plants · 2024Review
- HEI10 is subject to phase separation and mediates RPA1a degradation during meiotic interference-sensitive crossover formation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Arabidopsis lamin-like proteins CRWN1 and CRWN2 interact with SUPPRESSOR OF NPR1-1 INDUCIBLE 1 and RAD51D to prevent DNA damage.The Plant cell · 2023Article
- The ATR-WEE1 kinase module promotes SUPPRESSOR OF GAMMA RESPONSE 1 translation to activate replication stress responses.The Plant cell · 2023Article
- The SMC5/6 complex recruits the PAF1 complex to facilitate DNA double-strand break repair in Arabidopsis.The EMBO journal · 2023Article
- APC/CCDC20 targets SCFFBL17 to activate replication stress responses in Arabidopsis.The Plant cell · 2023Article
- How Do Plants Cope with DNA Damage? A Concise Review on the DDR Pathway in Plants.International journal of molecular sciences · 2023Review
- RNA-binding protein MAC5A interacts with the 26S proteasome to regulate DNA damage response in Arabidopsis.Plant physiology · 2023Article
- MDF is a conserved splicing factor and modulates cell division and stress response inLife science alliance · 2023Article
- A diRNA-protein scaffold module mediates SMC5/6 recruitment in plant DNA repair.The Plant cell · 2022Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA damage response is a fundamental mechanism to maintain genome stability. The ATR-WEE1 kinase module plays a central role in response to replication stress. Although the ATR-WEE1 pathway has been well studied in yeasts and animals, how ATR-WEE1 functions in plants remains unclear. Through a genetic screen for suppressors of the Arabidopsis atr mutant, we found that loss of function of PRL1, a core subunit of the evolutionarily conserved MAC complex involved in alternative splicing, suppresses the hypersensitivity of atr and wee1 to replication stress. Biochemical studies revealed that WEE1 directly interacts with and phosphorylates PRL1 at Serine 145, which promotes PRL1 ubiquitination and subsequent degradation. In line with the genetic and biochemical data, replication stress induces intron retention of cell cycle genes including CYCD1;1 and CYCD3;1, which is abolished in wee1 but restored in wee1 prl1. Remarkably, co-expressing the coding sequences of CYCD1;1 and CYCD3;1 partially restores the root length and HU response in wee1 prl1. These data suggested that the ATR-WEE1 module inhibits the MAC complex to regulate replication stress responses. Our study discovered PRL1 or the MAC complex as a key downstream regulator of the ATR-WEE1 module and revealed a novel cell cycle control mechanism.
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