ArticlePloS one2021
Methionine supplementing effects on intestine, liver and uterus morphology, and on positivity and expression of Calbindin-D28k and TRPV6 epithelial calcium carriers in laying quail in thermoneutral conditions and under thermal stress.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 23 citations in OpenAlex.
- Effects of a multi-strain probiotic on production performance, antioxidant indices, intestinal morphology, and fecal microbiota in heat-stressed quails.Tropical animal health and production · 2026Article
- Effects of Different Levels of Methionine in Liquid and Powder Forms on Growth Performance, Blood Parameters, and Carcass Traits of Broiler Chickens.Veterinary medicine and science · 2026Article
- Article
- Heat Stress in Japanese Quails (Animals : an open access journal from MDPI · 2024Review
- Exploration of Some Nutrient Absorption-Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive Lifespan.Veterinary medicine and science · 2024Article
- Dietary supplementation with calcitriol or quercetin improved eggshell and bone quality by modulating calcium metabolism.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- Effect of heat stress on the hypothalamic expression profile of water homeostasis-associated genes in low- and high-water efficient chicken lines.Physiological reports · 2024Article
- Heat stress and poultry production: a comprehensive review.Poultry science · 2023Review
- Uterine inflammation status modulates eggshell mineralization via calcium transport and matrix protein synthesis in laying hens.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Article
- Rumen-protected zinc-methionine dietary inclusion alters dairy cow performances, and oxidative and inflammatory status under long-term environmental heat stress.Frontiers in veterinary science · 2022Article
- Effect of prenatal ambient temperature on the performance physiological parameters, and oxidative metabolism of Japanese quail (Coturnix coturnix japonica) layers exposed to heat stress during growth.Scientific reports · 2021Article
- Methionine Supplementation of Maternal Diet Improves Hatching Traits, Initial Development, and Performance in Japanese Quail Fed Different Levels of Methionine During Growth.Animal science journal = Nihon chikusan GakkaihoArticle
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aimed to provide the performance, localization and expression of the epithelial calcium transporter channels Calbindin-D28k (Calb) and TRPV6, and of the morphology of the digestive and reproductive system of laying quail under heat stress (HS), and with methionine supplementation (MS). This study characterized the positivity (immunohistochemistry) and expression (real-time PCR) of calcium channels in the kidneys, intestine and uterus of 504 laying quails under different MS (100, 110 and 120%) and temperatures (20, 24, 28 and 32°C). The animals under HS (32°C) had lower villus height, villus:crypt ratio, and goblet cell index in the duodenum and jejunum, fewer secondary and tertiary uterine folds, smaller hepatic steatosis, and increased number of distal convoluted renal tubules (CT) positive to Calb, and increased positivity in proximal CTs. Deleterious effects of HS were minimized with MS for: duodenal crypts, number of goblet cells of the jejunum, number of uterine folds, decreased Calb positivity in intestines and kidney, increased positivity of Calb in the uterus and increased TRPV6 gene expression in the kidney (P≤0.05). Epithelial calcium transporters were altered due to less need for calcium absorption and reabsorption due to more calcium available with the MS, increasing egg production in HS and quality in termoneutrality (P≤0.05). MS further increased intestinal villus absorption area and height, increased steatosis, decreased Calb positivity in the intestine and kidney, increased uterine positivity of Calb, and increase Calb and TRPV6 expression in the kidney (P≤0.001) under thermoneutrality. It was concluded that the use of MS (120%) is justifiable in order to partially reverse the deleterious effects of HS on the production, in the epithelial calcium carriers, and in the digestory and reproductive morphology of laying quail.
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