Evidence map›Paper›PMID 33449226›Full record

ArticleDigestive diseases and sciences2021

Circ_0004104 Accelerates the Progression of Gastric Cancer by Regulating the miR-539-3p/RNF2 Axis.

Furong Yue, Keyu Peng, Li Zhang, Jun Zhang

Abstract read
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In one paragraph

Article in Digestive diseases and sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.

  1. Pooled it
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  6. Article
  7. Implication of protein post translational modifications in gastric cancer.Frontiers in cell and developmental biology · 2025
    Review
  8. Article
  9. Article
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  12. Article
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  14. Article
  15. Gastric cancer: An epigenetic view.World journal of gastrointestinal oncology · 2022
    Review
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  17. Article
  18. Article
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  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Furong Yue *Department of Oncology, Chongqing Three Gorges Central Hospital and Chongqing University Three Gorges Hospital, No. 165, Xin Cheng Road, Wanzhou District, Chongqing, 404000, China.
Keyu Peng *Department of Oncology, Chongqing Three Gorges Central Hospital and Chongqing University Three Gorges Hospital, No. 165, Xin Cheng Road, Wanzhou District, Chongqing, 404000, China.
Li ZhangDepartment of Oncology, Chongqing Three Gorges Central Hospital and Chongqing University Three Gorges Hospital, No. 165, Xin Cheng Road, Wanzhou District, Chongqing, 404000, China.
Jun ZhangDepartment of Oncology, Chongqing Three Gorges Central Hospital and Chongqing University Three Gorges Hospital, No. 165, Xin Cheng Road, Wanzhou District, Chongqing, 404000, China. yuefurong2020@163.com.
Chongqing Three Gorges Central Hospital · CNChongqing University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCircular RNA (circRNA) has been shown to be closely associated with cancer progression, including gastric cancer (GC). However, the function of circ_0004104 in GC progression has not been clarified.

aimsThe purpose of this study was to explore the role of circ_0004104 in GC progression.

methodsThe expression levels of circ_0004104, miR-539-3p, and ring finger protein 2 (RNF2) were assessed using quantitative real-time polymerase chain reaction. Cell proliferation was measured by 3-(4,5-dimethyl-2 thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay, and cell migration and invasion were detected using transwell assay. The levels of glutamine, glutamate, and α-ketoglutarate were determined to evaluate the glutaminolysis of cells, and the protein levels of glutaminolysis-related markers and RNF2 were detected using western blot analysis. Furthermore, Dual-Luciferase Reporter Assay was employed to assess the interaction between miR-539-3p and circ_0004104 or RNF2. Animal experiments were carried out to evaluate the effect of circ_0004104 silencing on GC tumor growth in vivo.

resultsCirc_0004104 was upregulated in GC, and its knockdown repressed the proliferation, metastasis, and glutaminolysis of GC cells in vitro and reduced GC tumor growth in vivo. Furthermore, we discovered that circ_0004104 could sponge miR-539-3p and miR-539-3p could target RNF2. The rescue experiments suggested that miR-539-3p inhibitor could reverse the suppressive effect of circ_0004104 silencing on GC progression, and RNF2 overexpression also reversed the inhibition effect of miR-539-3p mimic on GC progression.

conclusionCirc_0004104 accelerated GC progression via regulating the miR-539-3p/RNF2 axis, indicating that circ_0004104 might be a potential therapeutic target for GC.

Indexed as

AgedAnimalsCell Line, TumorCell MovementCell ProliferationDisease ProgressionExosomesFemaleGene Expression Regulation, NeoplasticHumansMaleMiceMice, Inbred BALB CMice, NudeMicroRNAsMiddle AgedMicroRNAsMIRN539 microRNA, humanPolycomb Repressive Complex 1RNA, CircularRNF2 protein, humanCirc_0004104Gastric cancermiR-539-3pRNF2

Identifiers

PMID33449226
OpenAlexW3122092578

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.