ArticlePlant cell reports2021
Targeted editing of tomato carotenoid isomerase reveals the role of 5' UTR region in gene expression regulation.
Article in Plant cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Genome Editing in Solanaceae: Harnessing CRISPR-Cas Technology for Precision Crop Improvement.Plants (Basel, Switzerland) · 2026Review
- Discovery of major QTL and a massive haplotype associated with cannabinoid biosynthesis in drug-type Cannabis.The plant genome · 2025Article
- Review
- Unleashing the Potential of CRISPR/Cas9 Genome Editing for Yield-Related Traits in Rice.Plants (Basel, Switzerland) · 2024Review
- Application of genome editing techniques to regulate gene expression in crops.BMC plant biology · 2024Review
- Synthetic directed evolution for targeted engineering of plant traits.Frontiers in plant science · 2024Review
- Characterization of prolycopene-accumulated TanJournal of biosciences · 2023Article
- CRISPR/Cas genome editing in tomato improvement: Advances and applications.Frontiers in plant science · 2023Review
- In-Depth Characterization ofFrontiers in plant science · 2022Article
- Nucleotide variation in the phytoene synthase (ClPsy1) gene contributes to golden flesh in watermelon (Citrullus lanatus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- The Genetic Components of a Natural Color Palette: A Comprehensive List of Carotenoid Pathway Mutations in Plants.Frontiers in plant science · 2021Review
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageA deletion created by CRISPR/Cas9 system in the 5' UTR of the carotenoid isomerase gene in tomato leads to downregulation of the gene resulting in the low conversion of prolycopene to lycopene. CRISPR/Cas9 based genome editing is an effective and useful tool adopted from the bacterial immune response system for altering specific, pre-determined DNA sequences in eukaryotes. Such targeted changes are finding wide application in human health as well as in precision breeding of crop plants for improved traits. Mutations in the coding and regulatory regions can have varying impacts on the function of the gene. In the current study, we demonstrate this on tomato carotenoid isomerase, a key gene in the carotenoid biosynthesis pathway. Mutations were generated in the 5' UTR and exon 1 of the carotenoid isomerase gene using CRISPR/Cas9 expression via Agrobacterium-mediated transformation of tomato variety Periyakulam 1 (PKM1). Molecular and biochemical studies demonstrate that CRISPR-mediated point mutations in the exon sequence lead to complete knockout of protein function whereas deletion in 5' UTR region lowers the expression of the gene leading to changes in plant phenotype.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.