ArticleeLife2021
Bi-fated tendon-to-bone attachment cells are regulated by shared enhancers and KLF transcription factors.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 60 citations in OpenAlex.
- Piezo1 channel: structure, mechanogating mechanism, functions, diseases and therapeutic strategy.Molecular biomedicine · 2026Review
- The developing tendon and enthesis are hypoxic and rely on hypoxia-inducible factor 1a during postnatal development.Development (Cambridge, England) · 2026Article
- Single-cell transcriptomics-informed induced pluripotent stem cells differentiation to tenogenic lineage.eLife · 2026Article
- Identification of a new population of TnnBone research · 2026Article
- Article
- Analysis of cranial tenocyte heterogeneity reveals a role for Wnt signaling in tendon attachments.Development (Cambridge, England) · 2026Article
- The developing tendon and enthesis are hypoxic and rely on hypoxia-inducible factor 1a (bioRxiv : the preprint server for biology · 2025Article
- Mesenchymal stem cell activity across a graded scaffold-hydrogel composite biomaterial for tendon-to-bone enthesis repair.Bioactive materials · 2025Article
- A Cold-Active Protease Tissue Dissociation Protocol for the Preservation of the Tendon Fibroblast Transcriptome.Bio-protocol · 2025Article
- Article
- Il-6 signaling exacerbates hallmarks of chronic tendon disease by stimulating reparative fibroblasts.eLife · 2025Article
- Paracrine signals influence patterns of fibrocartilage differentiation in a lyophilized gelatin hydrogel for applications in rotator cuff repair.Biomaterials science · 2024Article
- Pre-hypertrophic chondrogenic enhancer landscape of limb and axial skeleton development.Nature communications · 2024Article
- NSUN2 facilitates tenogenic differentiation of rat tendon-derived stem cells via m5C methylation of KLF2.Regenerative therapy · 2024Article
- KLF transcription factors in bone diseases.Journal of cellular and molecular medicine · 2024Review
- Interfacial Tissue Regeneration with Bone.Current osteoporosis reports · 2024Review
- Article
- Mechanical force regulates Sox9 expression at the developing enthesis.Development (Cambridge, England) · 2023Article
- Single-cell and spatial transcriptomics reveal changes in cell heterogeneity during progression of human tendinopathy.BMC biology · 2023Article
- Single-cell RNA sequencing in orthopedic research.Bone research · 2023Review
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Authors and funding
16 authors at 4 institutions in 3 countries.
Funding
Abstract
The mechanical challenge of attaching elastic tendons to stiff bones is solved by the formation of a unique transitional tissue. Here, we show that murine tendon-to-bone attachment cells are bi-fated, activating a mixture of chondrocyte and tenocyte transcriptomes, under regulation of shared regulatory elements and Krüppel-like factors (KLFs) transcription factors. High-throughput bulk and single-cell RNA sequencing of humeral attachment cells revealed expression of hundreds of chondrogenic and tenogenic genes, which was validated by in situ hybridization and single-molecule ISH. ATAC sequencing showed that attachment cells share accessible intergenic chromatin areas with either tenocytes or chondrocytes. Epigenomic analysis revealed enhancer signatures for most of these regions. Transgenic mouse enhancer reporter assays verified the shared activity of some of these enhancers. Finally, integrative chromatin and motif analyses and transcriptomic data implicated KLFs as regulators of attachment cells. Indeed, blocking expression of both
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