ArticleACS applied materials & interfaces2021
Immobilization Strategies for Enhancing Sensitivity of Electrochemical Aptamer-Based Sensors.
Article in ACS applied materials & interfaces, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Freeze-thaw-directed interfacial assembly of a gold nanoparticle/MXene electrochemical aptamer sensor for antibody-free myeloperoxidase detection.Mikrochimica acta · 2026Article
- Tunable Osmium Complexes as pH-Insensitive Redox Reporters for Electrochemical Biosensing.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Novel Electrochemical Aptasensor Based on Iron-Cobalt-Doped Magnetic Carbon and cDNA-Polyacrylic Acid for the Determination of Aflatoxin B1 in Peanuts.Sensors (Basel, Switzerland) · 2026Article
- Biofunctionalized polymer semiconductors toward soft and stretchable transistor-based biosensors.Science advances · 2026Article
- Beyond Self-Assembly: Bioorthogonal 'Click' Chemistry Strategies for Robust Electrochemical Interfaces in Wearable Biosensors.Biosensors · 2026Review
- A proof-of-concept study using a graphene oxide-bovine serum albumin nanocomposite-based electrochemical aptasensor platform forThe veterinary quarterly · 2025Article
- Simultaneous voltammetric detection of clenbuterol and ractopamine using violet phosphorene-MWCNT nanocomposite sensor optimized by multi-objective particle swarm algorithm.Mikrochimica acta · 2025Article
- On the Design, Fabrication, and Operation of Electrochemical Aptamer-Based Sensors.ACS sensors · 2025Review
- Designing the Future of Biosensing: Advances in Aptamer Discovery, Computational Modeling, and Diagnostic Applications.Biosensors · 2025Review
- Electrochemical Impedance Spectroscopy-Based Biosensors for Label-Free Detection of Pathogens.Biosensors · 2025Review
- Development and synthesis of DNA-based label-free electrochemical biosensor for detection of Enterocytozoon bieneusi using screen-printed gold electrode.BMC chemistry · 2025Article
- Strategic Detection ofBiosensors · 2025Review
- Review
- Tandem metabolic reaction-based sensors unlock in vivo metabolomics.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Carboxylate-Terminated Electrode Surfaces Improve the Performance of Electrochemical Aptamer-Based Sensors.ACS applied materials & interfaces · 2025Article
- Electrochemical aptasensor for the selective detection of vancomycin based on nanostructured "in-lab" printed electrodes.Mikrochimica acta · 2025Article
- Biosensors for Monitoring, Detecting, and Tracking Dissemination of Poultry-Borne Bacterial Pathogens Along the Poultry Value Chain: A Review.Animals : an open access journal from MDPI · 2024Review
- A Novel Methylene Blue Indicator-Based Aptasensor for Rapid Detection ofInternational journal of molecular sciences · 2024Article
- Electrocatalysis in MOF Films for Flexible Electrochemical Sensing: A Comprehensive Review.Biosensors · 2024Review
- Measurement of Neuropeptide Y in Aptamer-Modified Planar Electrodes.Electrochimica acta · 2024Article
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Authors and funding
4 authors.
Funding
Abstract
Electrochemical aptamer-based (E-AB) sensors are a versatile sensing platform that can achieve rapid and robust target detection in complex matrices. However, the limited sensitivity of these sensors has impeded their translation from proof-of-concept to commercial products. Surface-bound aptamers must be sufficiently spaced to bind targets and subsequently fold for signal transduction. We hypothesized that electrodes fabricated using conventional methods result in sensing surfaces where only a fraction of aptamers are appropriately spaced to actively respond to the target. As an alternative, we presented a novel aptamer immobilization approach that favors sufficient spacing between aptamers at the microscale to achieve optimal target binding, folding, and signal transduction. We first demonstrated that immobilizing aptamers in their target-bound, folded state on gold electrode surfaces yields an aptamer monolayer that supports greater sensitivity and higher signal-to-noise ratio than traditionally prepared E-AB sensors. We also showed that performing aptamer immobilization under low ionic strength conditions rather than conventional high ionic strength buffer greatly improves E-AB sensor performance. We successfully tested our approach with three different small-molecule-binding aptamers, demonstrating its generalizability. On the basis of these results, we believe our electrode fabrication approach will accelerate development of high-performance sensors with the sensitivity required for real-world analytical applications.
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