ArticlePlant biotechnology journal2021
Metabolic engineering of rice endosperm towards higher vitamin B1 accumulation.
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- Reconfiguring biofortification strategies to transform food systems and address micronutrient deficiency of the 21st century.Journal of integrative plant biology · 2026Review
- ZmTH1 Is Vital for Healthy Plant Growth and Promotes Cold/Drought Tolerance by Regulating Thiamin Diphosphate-Dependent Metabolisms in Maize.Plant biotechnology journal · 2026Article
- Engineering hormonal crosstalk to enhance serotonin/melatonin levels in rice.Nature communications · 2025Article
- Plant-derived vesicle-like nanoparticles in food crops: emerging insights into nutritional biofortification and biomedical applications.Plant biotechnology journal · 2025Review
- Plant synthetic biology-based biofortification, strategies and recent progresses.Journal of integrative plant biology · 2025Review
- Navigating the challenges of engineering composite specialized metabolite pathways in plants.The Plant journal : for cell and molecular biology · 2025Review
- In silico, in vitro, and in vivo characterization of thiamin-binding proteins from plant seeds.The Biochemical journal · 2025Article
- Ectopic biosynthesis of catechin of tea plant can be completed by co-expression of the threeHorticulture research · 2025Article
- Effect of Degree of Milling (DOM) on Physicochemical and Nutritional Quality of Selected Rice Variety (BRRI dhan78).International journal of food science · 2025Article
- OsTH1 is a key player in thiamin biosynthesis in rice.Scientific reports · 2024Article
- A protocol for a turbidimetric assay using a Saccharomyces cerevisiae thiamin biosynthesis mutant to estimate total vitamin BPlant methods · 2023Article
- Biofortification: an approach to eradicate micronutrient deficiency.Frontiers in nutrition · 2023Review
- Functional and comparative analysis ofPeerJ · 2023Article
- B vitamin supply in plants and humans: the importance of vitamer homeostasis.The Plant journal : for cell and molecular biology · 2022Review
- A novel panel of yeast assays for the assessment of thiamin and its biosynthetic intermediates in plant tissues.The New phytologist · 2022Article
- Review
- Natural Variation in Vitamin BFrontiers in plant science · 2022Article
- Variation of vitamin B contents in maize inbred lines: Potential genetic resources for biofortification.Frontiers in nutrition · 2022Article
- Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants.Plant physiology · 2021Article
- Riboflavin fortification of rice endosperm by metabolic engineering.Plant biotechnology journal · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rice is a major food crop to approximately half of the human population. Unfortunately, the starchy endosperm, which is the remaining portion of the seed after polishing, contains limited amounts of micronutrients. Here, it is shown that this is particularly the case for thiamin (vitamin B1). Therefore, a tissue-specific metabolic engineering approach was conducted, aimed at enhancing the level of thiamin specifically in the endosperm. To achieve this, three major thiamin biosynthesis genes, THIC, THI1 and TH1, controlled by strong endosperm-specific promoters, were employed to obtain engineered rice lines. The metabolic engineering approaches included ectopic expression of THIC alone, in combination with THI1 (bigenic) or combined with both THI1 and TH1 (trigenic). Determination of thiamin and thiamin biosynthesis intermediates reveals the impact of the engineering approaches on endosperm thiamin biosynthesis. The results show an increase of thiamin in polished rice up to threefold compared to WT, and stable upon cooking. These findings confirm the potential of metabolic engineering to enhance de novo thiamin biosynthesis in rice endosperm tissue and aid in steering future biofortification endeavours.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.