Evidence map›Paper›PMID 33447822›Full record

ArticleBiofilm2020

Utilizing glycoside hydrolases to improve the quantitation and visualization of biofilm bacteria.

Derek Fleming, Whitni Redman, Garrett S Welch, Nontokozo V Mdluli, Candace N Rouchon, Kristi L Frank, Kendra P Rumbaugh

Open access · goldAbstract read
In one paragraph

Article in Biofilm, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Biofilm dispersion inbioRxiv : the preprint server for biology · 2026
    Article
  3. Article
  4. Article
  5. Biochemistry and biophysics reports · 2025
    Article
  6. Review
  7. Article
  8. Contribution ofFrontiers in cellular and infection microbiology · 2022
    Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Derek FlemingDepartments of Surgery, Immunology and Molecular Microbiology, and the Burn Center of Research Excellence, Texas Tech University Health Sciences Center, Lubbock, TX, USA.
Whitni RedmanDepartments of Surgery, Immunology and Molecular Microbiology, and the Burn Center of Research Excellence, Texas Tech University Health Sciences Center, Lubbock, TX, USA.
Garrett S WelchDepartments of Surgery, Immunology and Molecular Microbiology, and the Burn Center of Research Excellence, Texas Tech University Health Sciences Center, Lubbock, TX, USA.
Nontokozo V MdluliDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Candace N RouchonDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Kristi L FrankDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Kendra P RumbaughDepartments of Surgery, Immunology and Molecular Microbiology, and the Burn Center of Research Excellence, Texas Tech University Health Sciences Center, Lubbock, TX, USA.
Texas Tech University · USUniformed Services University of the Health Sciences · US

Funding

Role of the enterococcal site 2 protease in biofilm formation, adaptation, and host-pathogen interactionsR01AI141961 · NIAID · HENRY M. JACKSON FDN FOR THE ADV MIL/MED · PI FRANK, KRISTI L · 2019 to 2023
$1.9M
Utilizing Glycoside Hydrolases to Degrade Biofilms in WoundsR21AI137462 · NIAID · TEXAS TECH UNIVERSITY HEALTH SCIS CENTER · PI RUMBAUGH, KENDRA P · 2019 to 2020
$286k
NIAID NIH HHS R01 AI141961NIAID NIH HHS R21 AI137462
6 · The paper itself

Abstract

The complexity of microbial biofilms offers several challenges to the use of traditional means of microbial research. In particular, it can be difficult to calculate accurate numbers of biofilm bacteria, because even after thorough homogenization or sonication, small pieces of the biofilm remain, which contain numerous bacterial cells and result in inaccurately low colony forming units (CFU). In addition, imaging of infected tissue

Identifiers

PMID33447822
PMCPMC7798457
OpenAlexW3082633901

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.