Evidence map›Paper›PMID 33447797›Full record

ArticleBiofilm2020

Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates.

Jontana Allkja, Thomas Bjarnsholt, Tom Coenye, Paul Cos, Adyary Fallarero, Joe J Harrison, Susana P Lopes, Antonio Oliver, Maria Olivia Pereira, Gordon Ramage and 6 more

Abstract read
In one paragraph

Article in Biofilm, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. A systematic review of the use of bacteriophages for in vitro biofilm control.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2023
    Pooled it
  2. Review
  3. Review
  4. Review
  5. Phenotypic and Genomic Analysis of Swine-DerivedAnimals : an open access journal from MDPI · 2026
    Article
  6. Review
  7. Article
  8. Biofilm Formation inMicroorganisms · 2026
    Article
  9. Microbial and host innate immune factors affecting the persistence ofFrontiers in cellular and infection microbiology · 2026
    Review
  10. Review
  11. Observational
  12. Article
  13. Article
  14. Article
  15. Anti-Biofilm Agents to OvercomePharmaceuticals (Basel, Switzerland) · 2025
    Review
  16. Homotypic Membrane Vesicle-Formulated VAN@Biomaterials research · 2025
    Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Jontana AllkjaLEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465, Porto, Portugal.
Thomas BjarnsholtDepartment of Clinical Microbiology, Rigshospitalet, 2100, Copenhagen, Denmark.
Tom CoenyeESCMID Study Group for Biofilms, Basel, Switzerland.
Paul CosLaboratory for Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp, Belgium.
Adyary FallareroPharmaceutical Design and Discovery (PharmDD), Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, Viikinkaari 5E, P.O. Box 56, FI-00014, Helsinki, Finland.
Joe J HarrisonDepartment of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N 1N4, Canada.
Susana P LopesCentre of Biological Engineering (CEB), Laboratório de Investigação Em Biofilmes Rosário Oliveira (LIBRO), University of Minho, Braga, Portugal.
Antonio OliverServicio de Microbiología, Hospital Universitario Son Espases, Instituto de Investigación Sanitaria Illes Balears (IdISBa), Palma de Mallorca, Spain.
Maria Olivia PereiraCentre of Biological Engineering (CEB), Laboratório de Investigação Em Biofilmes Rosário Oliveira (LIBRO), University of Minho, Braga, Portugal.
Gordon RamageOral Sciences Research Group, University of Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom.
Mark E ShirtliffDepartment of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, MD, 21201, USA.
Paul StoodleyDepartment of Microbial Infection and Immunity and Orthopedics, The Ohio State University, Columbus, OH, 43210, USA.
Jeremy S WebbNational Biofilms Innovation Centre, School of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK.
Sebastian A J ZaatAmsterdam UMC, University of Amsterdam, Department of Medical Microbiology, Amsterdam Infection and Immunity Institute, Meibergdreef 9, 1105AZ, Amsterdam, the Netherlands.
Darla M GoeresMontana State University, Center for Biofilm Engineering, 366 Barnard Hall, Bozeman, MT, 59717, USA.
Nuno Filipe AzevedoLEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465, Porto, Portugal.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The lack of reproducibility of published studies is one of the major issues facing the scientific community, and the field of biofilm microbiology has been no exception. One effective strategy against this multifaceted problem is the use of minimum information guidelines. This strategy provides a guide for authors and reviewers on the necessary information that a manuscript should include for the experiments in a study to be clearly interpreted and independently reproduced. As a result of several discussions between international groups working in the area of biofilms, we present a guideline for the spectrophotometric and fluorometric assessment of biofilm formation in microplates. This guideline has been divided into 5 main sections, each presenting a comprehensive set of recommendations. The intention of the minimum information guideline is to improve the quality of scientific communication that will augment interlaboratory reproducibility in biofilm microplate assays.

Indexed as

BiofilmFluorometryGuidelinesMicroplateReproducibilitySpectrophotometry

Identifiers

PMID33447797
PMCPMC7798448

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.