ArticleOpen forum infectious diseases2021
Performance of a Semiquantitative Multiplex Bacterial and Viral PCR Panel Compared With Standard Microbiological Laboratory Results: 396 Patients Studied With the BioFire Pneumonia Panel.
Article in Open forum infectious diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Identification of bacterial co-detections in COVID-19 critically Ill patients by BioFire® FilmArray® pneumonia panel: a systematic review and meta-analysis.Diagnostic microbiology and infectious disease · 2021Pooled it
- Trial
- Diagnostic utility of oropharyngeal swabs as an alternative to lower respiratory tract samples for PCR-based syndromic testing in patients with community-acquired pneumonia.Journal of clinical microbiology · 2023Trial
- Faster Diagnosis of Suspected Lower Respiratory Tract Infections: Single-Center Evidence from BIOFIRE FilmArrayDiagnostics (Basel, Switzerland) · 2026Article
- Performance of BIOFIRE FILMARRAY pneumonia panel in suspected pneumonia: insights from a real-world study.Microbiology spectrum · 2025Article
- Pneumonia: Recent Updates on Diagnosis and Treatment.Microorganisms · 2025Review
- Multicenter evaluation of fast multiplex PCR for detection of pathogens in lower respiratory tract infections.Frontiers in cellular and infection microbiology · 2025Observational
- The Impact of Pneumonia PCR Panel Testing in the PICU: A Quality Improvement Study.Journal of pediatric intensive care · 2024Article
- Utility of a multiplex pathogen detection system directly from respiratory specimens for treatment and diagnostic stewardship.Microbiology spectrum · 2024Article
- Breaking Boundaries in Pneumonia Diagnostics: Transitioning from Tradition to Molecular Frontiers with Multiplex PCR.Diagnostics (Basel, Switzerland) · 2024Review
- Evaluation of the clinical relevance of the BiofireInfection · 2024Article
- Real-life Assessment of BioFire FilmArray Pneumonia Panel in Adults Hospitalized With Respiratory Illness.The Journal of infectious diseases · 2024Article
- Characteristics, Outcomes, and Factors Affecting Mortality in Hospitalized Patients with CAP Due to Different Variants of SARS-CoV-2 and Non-COVID-19 CAP.Journal of clinical medicine · 2023Article
- Value of sputum Gram stain, sputum culture, and bronchoalveolar lavage fluid Gram stain in predicting single bacterial pathogen among children with community-acquired pneumonia.BMC pulmonary medicine · 2022Article
- BioFire FilmArray Pneumonia Panel enhances detection of pathogens and antimicrobial resistance in lower respiratory tract specimens.Annals of clinical microbiology and antimicrobials · 2022Article
- Comparison of a Point-of-Care FilmArray Test to Standard-of-Care Microbiology Test in Diagnosis of Healthcare Associated Infections in a Tertiary Care Pediatric Intensive Care Unit.Antibiotics (Basel, Switzerland) · 2022Article
- Article
- Rapid syndromic PCR testing in patients with respiratory tract infections reduces time to results and improves microbial yield.Scientific reports · 2022Article
- A multiplex pneumonia panel for diagnosis of hospital-acquired and ventilator-associated pneumonia in the era of emerging antimicrobial resistance.Frontiers in cellular and infection microbiology · 2022Article
- Relationship of Multiplex Molecular Pneumonia Panel Results With Hospital Outcomes and Clinical Variables.Open forum infectious diseases · 2021Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicrobiologic results are critical to optimal management of patients with lower respiratory tract infection, but standard methods may take several days. The multiplex polymerase chain reaction BioFire Pneumonia (PN) panel detects 15 common bacterial species semiquantitatively as copy number/mL, 8 viral species, and 7 resistance genes in about an hour within the clinical laboratory.
methodsWe tested 396 unique endotracheal or bronchoalveolar lavage specimens with the BioFire Pneumonia panel and compared the bacterial detections to conventional gram stain and culture results.
resultsOf the 396 patients, 138 grew at least 1 bacterium that had a target on the PN panel, and 136/138 (98.6%) were detected by the panel. A total of 177 isolates were recovered in culture and the PN panel detected 174/177 (98.3%). A further 20% of patients had additional targets detected that were not found on standard culture (specificity 69%, positive predictive value 63%, and negative predictive value 98.9%). Copy number was strongly related to standard semiquantitative growth on plates reported by the laboratory (eg, 1+, 2+, 3+ growths) and was significantly higher in those specimens that grew a potential pathogen. Both higher copy number and bacterial detections found by the PN panel, but not found in culture, were strongly positively related to the level of white blood cells reported in the initial gram stain.
conclusionsHigher copy number and bacterial detections by the PN panel are related to the host respiratory tract inflammatory response. If laboratories can achieve a rapid turnaround time, the PN panel should have a significant impact both on patient management and on antibiotic stewardship.
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