Evidence map›Paper›PMID 33446811›Full record

ArticleScientific reports2021

Non-invasive synchronous monitoring of neutrophil migration using whole body near-infrared fluorescence-based imaging.

Jack Leslie, Stuart M Robinson, Fiona Oakley, Saimir Luli

Abstract read
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Recent advances in non-invasiveBiomaterials science · 2025
    Review
  3. Article
  4. Article
  5. Outer membrane vesicles ofMicrobiology spectrum · 2024
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jack LeslieNewcastle Fibrosis Research Group, Faculty of Medical Sciences, Biosciences Institute, Newcastle University, Newcastle upon Tyne, UK.
Stuart M RobinsonNewcastle Fibrosis Research Group, Faculty of Medical Sciences, Biosciences Institute, Newcastle University, Newcastle upon Tyne, UK.
Fiona OakleyNewcastle Fibrosis Research Group, Faculty of Medical Sciences, Biosciences Institute, Newcastle University, Newcastle upon Tyne, UK.
Saimir LuliNewcastle Fibrosis Research Group, Faculty of Medical Sciences, Biosciences Institute, Newcastle University, Newcastle upon Tyne, UK. saimir.luli@newcastle.ac.uk.

Funding

Cancer Research UK 23390Medical Research Council MR/K001949/1Medical Research Council MR/R023026/1Wellcome Trust
6 · The paper itself

Abstract

Advances in fluorescence imaging coupled with the generation of near infrared probes have significantly improved the capabilities of non-invasive, real-time imaging in whole animals. In this study we were able to overcome a limitation of in vivo fluorescence imaging and have established a dual cell tracking method where two different cell types can be monitored according to the spectral signature of the cell labelling fluorophore. Using a mouse model of acute liver injury, we have characterised the in vivo migration patterns of wild type and transgenic neutrophils with impaired chemotaxis. Here, we were able to demonstrate that IVIS provides a sensitive multiplexing technology to differentiate two different cell populations based on the spectral signature of the cell labelling fluorophores. This spectral unmixing methodology has the potential to uncover multidimensional cellular interactions involved in many diseases such as fibrosis and cancer. In vivo spectral un-mixing provides a useful tool for monitoring multiple biological process in real-time in the same animal.

Indexed as

Cell MovementCell TrackingNeutrophilsAnimalsFluorescent DyesMiceMice, KnockoutMicroscopy, FluorescenceFluorescent Dyes

Identifiers

PMID33446811
PMCPMC7809207

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.