ArticleScientific reports2021
Autophagy facilitates type I collagen synthesis in periodontal ligament cells.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- 2-Hydroxyethyl Methacrylate Increases MMP-1 and MMP-3 Production in Human Gingival Fibroblasts.Journal of biochemical and molecular toxicology · 2026Article
- Non-Invasive Physical Plasma Regulates COX-2 via EP2/EP4 Expression in Periodontal Ligament Cells Under Inflammatory Conditions.International journal of molecular sciences · 2026Article
- Palmitic Acid Alters Longitudinal Bone Growth While Enhancing Matrix Maturation in an Organotypic Bone Model.Biomolecules · 2026Article
- The Role of Cellular Senescence in Oral Health and Disease.International journal of molecular sciences · 2026Review
- Autophagy in Acute Lung Injury.Cell biochemistry and biophysics · 2025Review
- Exploring the mechanical and biological interplay in the periodontal ligament.International journal of oral science · 2025Review
- Lysosomes as coordinators of cellular catabolism, metabolic signalling and organ physiology.Nature reviews. Molecular cell biology · 2024Review
- The Potential of Collagen Treatment for Comorbid Diseases.Polymers · 2023Review
- Restoration of mitochondrial function byiScience · 2023Article
- Article
- Therapeutic effects of peptide P140 in a mouse periodontitis model.Cellular and molecular life sciences : CMLS · 2022Article
- Article
- Autophagy Regulates Osteogenic Differentiation of Human Periodontal Ligament Stem Cells Induced by Orthodontic Tension.Stem cells international · 2022Article
- A functional outside-in signaling network of proteoglycans and matrix molecules regulating autophagy.Matrix biology : journal of the International Society for Matrix Biology · 2021Article
- Cordycepin-induced Keratinocyte Secretome Promotes Skin Cell Regeneration.In vivo (Athens, Greece)Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autophagy is a lysosomal protein degradation system in which the cell self-digests its intracellular protein components and organelles. Defects in autophagy contribute to the pathogenesis of age-related chronic diseases, such as myocardial infarction and rheumatoid arthritis, through defects in the extracellular matrix (ECM). However, little is known about autophagy in periodontal diseases characterised by the breakdown of periodontal tissue. Tooth-supportive periodontal ligament (PDL) tissue contains PDL cells that produce various ECM proteins such as collagen to maintain homeostasis in periodontal tissue. In this study, we aimed to clarify the physiological role of autophagy in periodontal tissue. We found that autophagy regulated type I collagen synthesis by elimination of misfolded proteins in human PDL (HPDL) cells. Inhibition of autophagy by E-64d and pepstatin A (PSA) or siATG5 treatment suppressed collagen production in HPDL cells at mRNA and protein levels. Immunoelectron microscopy revealed collagen fragments in autolysosomes. Accumulation of misfolded collagen in HPDL cells was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. E-64d and PSA treatment suppressed and rapamycin treatment accelerated the hard tissue-forming ability of HPDL cells. Our findings suggest that autophagy is a crucial regulatory process that facilitates type I collagen synthesis and partly regulates osteoblastic differentiation of PDL cells.
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