ArticleScientific reports2021
Deuterium-labeled Raman tracking of glucose accumulation and protein metabolic dynamics in Aspergillus nidulans hyphal tips.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
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- Visualization of a Bruton's Tyrosine Kinase Inhibitor Using Fluorescence and Raman Microscopy.Analytical chemistry · 2026Article
- Rapid Antifungal Susceptibility Testing by Stimulated Raman Photothermal Imaging of DAnalytical chemistry · 2026Article
- Vibrational spectroscopy methodology for profiling of mixed carbon metabolism in carotenogenic and oleaginous Mucor circinelloides.Microbial cell factories · 2026Article
- Ultrasmall episymbiont Nanosynbacter lyticus employs multiple ATP-generating metabolic pathways during horizontal transmission.The ISME journal · 2026Article
- Raman imaging of molecular groups in the wavenumber silent region.Analytical and bioanalytical chemistry · 2026Review
- Recent advances in Raman probes for multiplexed bioimaging.Inflammation and regeneration · 2025Review
- Illuminating life processes by vibrational probes.Nature methods · 2025Review
- Phylogeny-metabolism dual-directed single-cell genomics for dissecting and mining ecosystem function by FISH-scRACS-seq.Innovation (Cambridge (Mass.)) · 2025Article
- Integrated metabarcoding and culture-dependent assessments revealFrontiers in microbiology · 2025Article
- Innovative Approaches for Drug Discovery: Quantifying Drug Distribution and Response with Raman Imaging.Analytical chemistry · 2024Review
- Transcriptional heterogeneity of catabolic genes on the plasmid pCAR1 causes host-specific carbazole degradation.Applied and environmental microbiology · 2024Article
- Deuterium- and Alkyne-Based Bioorthogonal Raman Probes for In Situ Quantitative Metabolic Imaging of Lipids within Plants.JACS Au · 2023Article
- Hyperglycemia and cancer in human lung carcinoma by means of Raman spectroscopy and imaging.Scientific reports · 2022Article
- Mapping endocrine networks by stable isotope tracing.Current opinion in endocrine and metabolic research · 2022Review
- Raman Micro-spectroscopy and Imaging of Filamentous Fungi.Microbes and environments · 2022Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Filamentous fungi grow exclusively at their tips, where many growth-related fungal processes, such as enzyme secretion and invasion into host cells, take place. Hyphal tips are also a site of active metabolism. Understanding metabolic dynamics within the tip region is therefore important for biotechnology and medicine as well as for microbiology and ecology. However, methods that can track metabolic dynamics with sufficient spatial resolution and in a nondestructive manner are highly limited. Here we present time-lapse Raman imaging using a deuterium (D) tracer to study spatiotemporally varying metabolic activity within the hyphal tip of Aspergillus nidulans. By analyzing the carbon-deuterium (C-D) stretching Raman band with spectral deconvolution, we visualize glucose accumulation along the inner edge of the hyphal tip and synthesis of new proteins from the taken-up D-labeled glucose specifically at the central part of the apical region. Our results show that deuterium-labeled Raman imaging offers a broadly applicable platform for the study of metabolic dynamics in filamentous fungi and other relevant microorganisms in vivo.
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