Evidence map›Paper›PMID 33446694›Full record

ArticleScientific reports2021

ScreenSeed as a novel high throughput seed germination phenotyping method.

Nicolas Merieux, Pierre Cordier, Marie-Hélène Wagner, Sylvie Ducournau, Sophie Aligon, Dominique Job, Philippe Grappin, Edwin Grappin

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Nicolas Merieux *EffiSciency, ScreenSeed, Issy-les-Moulineaux, 97132, France.
Pierre Cordier *EffiSciency, ScreenSeed, Issy-les-Moulineaux, 97132, France.
Marie-Hélène WagnerGroupe d'Étude et de Contrôle des Variétés et des Semences (GEVES, Dept Seed Testing, Station Nationale d'Essais de Semences (SNES), 49071, Beaucouzé, France.
Sylvie DucournauGroupe d'Étude et de Contrôle des Variétés et des Semences (GEVES, Dept Seed Testing, Station Nationale d'Essais de Semences (SNES), 49071, Beaucouzé, France.
Sophie AligonInstitut de recherche en horticulture et semences (IRHS), UMR 1345 INRAE - Institut Agro - Université d'Angers, SFR 4207 QuaSav, 49071, Beaucouzé, France.
Dominique JobMicrobiologie, Adaptation et Pathogénie, UMR 5240 CNRS - INSA - Université Claude Bernard Lyon1 - Bayer CropScience, 69009, Lyon, France.
Philippe GrappinInstitut de recherche en horticulture et semences (IRHS), UMR 1345 INRAE - Institut Agro - Université d'Angers, SFR 4207 QuaSav, 49071, Beaucouzé, France. philippe.grappin@agrocampus-ouest.fr.
Edwin GrappinEffiSciency, ScreenSeed, Issy-les-Moulineaux, 97132, France. edwin.grappin@screenseed.com.
Institut de Recherche en Horticulture et Semences · FRInternational and European Law School · FRCentre National de la Recherche Scientifique · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A high throughput phenotyping tool for seed germination, the ScreenSeed technology, was developed with the aim of screening genotype responsiveness and chemical drugs. This technology was presently used with Arabidopsis thaliana seeds to allow characterizing seed samples germination behavior by incubating seeds in 96-well microplates under defined conditions and detecting radicle protrusion through the seed coat by automated image analysis. This study shows that this technology provides a fast procedure allowing to handle thousands of seeds without compromising repeatability or accuracy of the germination measurements. Potential biases of the experimental protocol were assessed through statistical analyses of germination kinetics. Comparison of the ScreenSeed procedure with commonly used germination tests based upon visual scoring displayed very similar germination kinetics.

Indexed as

Image Processing, Computer-AssistedArabidopsisGerminationSeeds

Identifiers

PMID33446694
PMCPMC7809209
OpenAlexW3125061028

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.