ArticleNature communications2021
Two distinct catalytic pathways for GH43 xylanolytic enzymes unveiled by X-ray and QM/MM simulations.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- A Cold- and Salt-Tolerant GH43-1 β-Xylosidase from Cellulomonas bogoriensis 69B4Applied biochemistry and biotechnology · 2026Article
- Genome Assembly and Genomic Characterization ofJournal of fungi (Basel, Switzerland) · 2026Article
- Whole-genome analysis and enzymatic profiling of Bacillus spizizenii as a xylanase-producing source from Iran.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Substrate-driven phage community structure and phage-host dynamics in anaerobic digesters.Environmental microbiome · 2026Article
- Multi-omics characterization of new and aged Daqu reveals region-specific microbial succession and metabolic signatures in Maotai-flavor liquor fermentation.Microbiology spectrum · 2026Article
- Conformational gating mechanism for processive catalysis of β(1,3)-glucans.Nature communications · 2026Article
- QM/MM Free Energy Calculations of IRE1 Reveal a Unique Protonation State of the Catalytic Lys599.Journal of computational chemistry · 2025Article
- Molecular insights of acarbose metabolization catalyzed by acarbose-preferred glucosidase.Nature communications · 2025Article
- Harnessing Xylanase Potential inJournal of fungi (Basel, Switzerland) · 2025Article
- Integration of Experimental and Computational Methods to Characterize Glycoproteins.Methods in molecular biology (Clifton, N.J.) · 2025Article
- An Unusual His/Asp Dyad Operates Catalysis in Agar-Degrading Glycosidases.ACS catalysis · 2024Article
- Thermophilic Hemicellulases Secreted by Microbial Consortia Selected from an Anaerobic Digester.International journal of molecular sciences · 2024Article
- Genome sequencing of Elaeocarpus spp. stem blight pathogen Pseudocryphonectria elaeocarpicola reveals potential adaptations to colonize woody bark.BMC genomics · 2024Article
- Lactate cross-feeding betweenApplied and environmental microbiology · 2024Article
- The evolutionary advantage of an aromatic clamp in plant family 3 glycoside exo-hydrolases.Nature communications · 2022Article
- Enzymatic Hydrolysis of Human Milk Oligosaccharides. The Molecular Mechanism ofACS catalysis · 2022Article
- Characterization of a GH Family 43 β-Xylosidase Having a Novel Carbohydrate-binding Module fromJournal of applied glycoscience · 2022Article
- Asparagine Tautomerization in Glycosyltransferase Catalysis. The Molecular Mechanism of ProteinACS catalysis · 2021Article
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10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Xylanolytic enzymes from glycoside hydrolase family 43 (GH43) are involved in the breakdown of hemicellulose, the second most abundant carbohydrate in plants. Here, we kinetically and mechanistically describe the non-reducing-end xylose-releasing exo-oligoxylanase activity and report the crystal structure of a native GH43 Michaelis complex with its substrate prior to hydrolysis. Two distinct calcium-stabilized conformations of the active site xylosyl unit are found, suggesting two alternative catalytic routes. These results are confirmed by QM/MM simulations that unveil the complete hydrolysis mechanism and identify two possible reaction pathways, involving different transition state conformations for the cleavage of xylooligosaccharides. Such catalytic conformational promiscuity in glycosidases is related to the open architecture of the active site and thus might be extended to other exo-acting enzymes. These findings expand the current general model of catalytic mechanism of glycosidases, a main reaction in nature, and impact on our understanding about their interaction with substrates and inhibitors.
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