ArticleEBioMedicine2021
A novel DNA chromatography method to discriminate Mycobacterium abscessus subspecies and macrolide susceptibility.
Article in EBioMedicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Comparative evaluation of molecular technologies for the identification of prevalent non-tuberculous mycobacteria in pulmonary infections: a systematic review and meta-analysis.Annals of medicine · 2026Pooled it
- Fallopian tube nontuberculous mycobacterial infection caused byCase reports in women's health · 2026Article
- Phylogenetic distribution and longitudinal persistence of plasmids inMicrobial genomics · 2026Article
- Comparison of culture and culture-free methods for comprehensive identification of mycobacteria: a single-center prospective study.Journal of clinical microbiology · 2026Article
- Successful treatment of inducible macrolide-resistantIDCases · 2026Article
- Antimicrobial resistance of rapidly growing mycobacteria isolated from companion animals in Taiwan.Microbiology spectrum · 2025Article
- Lipid fingerprinting by MALDI Biotyper Sirius instrument fails to differentiate the three subspecies of theJournal of clinical microbiology · 2025Article
- Targeted labeling and depletion of alveolar macrophages using VeDTR mouse technology.iScience · 2025Article
- Applications and advances in molecular diagnostics: revolutionizing non-tuberculous mycobacteria species and subspecies identification.Frontiers in public health · 2024Review
- Massive and Lengthy Clonal Nosocomial Expansion of Mycobacterium abscessus subsp.Microbiology spectrum · 2023Article
- Diagnostic Utility of a Mycobacterium Multiplex PCR Detection Panel for Tuberculosis and Nontuberculous Mycobacterial Infections.Microbiology spectrum · 2023Article
- MGIT-seq for the Identification of Nontuberculous Mycobacteria and Drug Resistance: a Prospective Study.Journal of clinical microbiology · 2023Article
- Antimicrobial Effect of Oxazolidinones and Its Synergistic Effect with Bedaquiline AgainstInfection and drug resistance · 2023Article
- Antibiotic-resistant status and pathogenic clonal complex of canine Streptococcus canis-associated deep pyoderma.BMC veterinary research · 2022Article
- Molecular Epidemiological Characteristics of Mycobacterium abscessus Complex Derived from Non-Cystic Fibrosis Patients in Japan and Taiwan.Microbiology spectrum · 2022Article
- The Use of Comparative Genomic Analysis for the Development of Subspecies-Specific PCR Assays forFrontiers in cellular and infection microbiology · 2022Article
- Quantitative evaluation of Mycobacterium abscessus clinical isolate virulence using a silkworm infection model.PloS one · 2022Article
- Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections.Microorganisms · 2021Review
- A first case report of nasopharyngeal Mycobacterium abscessus subspecies massiliense infection.European journal of medical research · 2021Article
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Authors and funding
15 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe clinical impact of infection with Mycobacterium (M.) abscessus complex (MABC), a group of emerging non-tuberculosis mycobacteria (NTM), is increasing. M. abscessus subsp. abscessus/bolletii frequently shows natural resistance to macrolide antibiotics, whereas M. abscessus subsp. massiliense is generally susceptible. Therefore, rapid and accurate discrimination of macrolide-susceptible MABC subgroups is required for effective clinical decisions about macrolide treatments for MABC infection. We aimed to develop a simple and rapid diagnostic that can identify MABC isolates showing macrolide susceptibility.
methodsWhole genome sequencing (WGS) was performed for 148 clinical or environmental MABC isolates from Japan to identify genetic markers that can discriminate three MABC subspecies and the macrolide-susceptible erm(41) T28C sequevar. Using the identified genetic markers, we established PCR based- or DNA chromatography-based assays. Validation testing was performed using MABC isolates from Taiwan. FINDING: We identified unique sequence regions that could be used to differentiate the three subspecies. Our WGS-based phylogenetic analysis indicated that M. abscessus carrying the macrolide-susceptible erm(41) T28C sequevar were tightly clustered, and identified 11 genes that were significantly associated with the lineage for use as genetic markers. To detect these genetic markers and the erm(41) locus, we developed a DNA chromatography method that identified three subspecies, the erm(41) T28C sequevar and intact erm(41) for MABC in a single assay within one hour. The agreement rate between the DNA chromatography-based and WGS-based identification was 99·7%.
interpretationWe developed a novel, rapid and simple DNA chromatography method for identification of MABC macrolide susceptibility with high accuracy.
fundingAMED, JSPS KAKENHI.
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