ArticleParasites & vectors2021
The bacterial community of the lone star tick (Amblyomma americanum).
Article in Parasites & vectors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Bacterial Communities Harboured by Amblyomma Hebraeum Infesting Small Stock in Mahikeng city, South Africa.Microbial ecology · 2025Article
- Genomic and Proteomic Analyses of Bacterial Communities ofMicroorganisms · 2025Article
- The Tick Microbiome: The "Other Bacterial Players" in Tick Biocontrol.Microorganisms · 2024Review
- The impact of bloodmeal and geographic region on the richness, diversity, and function of internal microbial community inHeliyon · 2024Article
- Metatranscriptomic investigation of singlemSystems · 2024Article
- Article
- The immune factors involved in the rapid clearance of bacteria from the midgut of the tickFrontiers in cellular and infection microbiology · 2024Article
- Diversity of the Bacterial and Viral Communities in the Tropical Horse Tick,Pathogens (Basel, Switzerland) · 2023Article
- Iva xanthiifolia leaf extract reduced the diversity of indigenous plant rhizosphere bacteria.BMC plant biology · 2023Article
- Characterization of bacterial communities in ticks parasitizing cattle in a touristic location in southwestern China.Experimental & applied acarology · 2023Article
- Diversity of the bacterial and viral communities in the tropical horse tick,bioRxiv : the preprint server for biology · 2023Article
- Comparison of salivary gland and midgut microbiome in the soft ticksFrontiers in microbiology · 2023Article
- Integrative analysis highlights molecular and immune responses of tickFrontiers in cellular and infection microbiology · 2023Article
- Identification and characterization of ecdysis-related neuropeptides in the lone star tickFrontiers in endocrinology · 2023Article
- Characterization and manipulation of the bacterial community in the midgut of Ixodes ricinus.Parasites & vectors · 2022Article
- Bacteria related to tick-borne pathogen assemblages in Ornithodoros cf. hasei (Acari: Argasidae) and blood of the wild mammal hosts in the Orinoquia region, Colombia.Experimental & applied acarology · 2022Article
- Hematophagy and tick-borne Rickettsial pathogen shape the microbial community structure and predicted functions within the tick vector,Frontiers in cellular and infection microbiology · 2022Article
- Article
- Recently EvolvedFrontiers in cellular and infection microbiology · 2022Article
- Grappling with the tick microbiome.Trends in parasitology · 2021Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe lone star tick (Amblyomma americanum), an important vector of a wide range of human and animal pathogens, is very common throughout the East and Midwest of the USA. Ticks are known to carry non-pathogenic bacteria that may play a role in their vector competence for pathogens. Several previous studies using the high throughput sequencing (HTS) technologies reported the commensal bacteria in a tick midgut as abundant and diverse. In contrast, in our preliminary survey of the field collected adult lone star ticks, we found the number of culturable/viable bacteria very low.
methodsWe aimed to analyze the bacterial community of A. americanum by a parallel culture-dependent and a culture-independent approach applied to individual ticks.
resultsWe analyzed 94 adult females collected in eastern Kansas and found that 60.8% of ticks had no culturable bacteria and the remaining ticks carried only 67.7 ± 42.8 colony-forming units (CFUs)/tick representing 26 genera. HTS of the 16S rRNA gene resulted in a total of 32 operational taxonomic units (OTUs) with the dominant endosymbiotic genera Coxiella and Rickettsia (> 95%). Remaining OTUs with very low abundance were typical soil bacterial taxa indicating their environmental origin.
conclusionsNo correlation was found between the CFU abundance and the relative abundance from the culture-independent approach. This suggests that many culturable taxa detected by HTS but not by culture-dependent method were not viable or were not in their culturable state. Overall, our HTS results show that the midgut bacterial community of A. americanum is very poor without a core microbiome and the majority of bacteria are endosymbiotic.
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