ArticleBiotechnology for biofuels2021
Analysis of the lipid extraction performance in a cascade process for Scenedesmus almeriensis biorefinery.
Article in Biotechnology for biofuels, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 37 citations in OpenAlex.
- Cascade Recovery of Bioactive Compounds from Spent Brewer's Yeast Using Pulsed Electric Field Treatment and Subsequent Enzymatic Hydrolysis.Microorganisms · 2026Article
- Biphasic lipid extraction from microalgae after PEF-treatment reduces the energy demand of the downstream process.Biotechnology for biofuels and bioproducts · 2025Article
- Simulation and Techno-Economical Evaluation of a Microalgal Biofertilizer Production Process.Biology · 2022Article
- Recovering Microalgal Bioresources: A Review of Cell Disruption Methods and Extraction Technologies.Molecules (Basel, Switzerland) · 2022Review
- Microalgal Biorefinery Concepts' Developments for Biofuel and Bioproducts: Current Perspective and Bottlenecks.International journal of molecular sciences · 2022Review
- Application of Pulsed Electric Fields and High-Pressure Homogenization in Biorefinery Cascade ofFoods (Basel, Switzerland) · 2022Article
- A Critical Review on Pulsed Electric Field: A Novel Technology for the Extraction of Phytoconstituents.Molecules (Basel, Switzerland) · 2021Review
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Authors and funding
9 authors at 2 institutions in 3 countries.
Funding
Abstract
backgroundMicroalgae have attracted considerable interest due to their ability to produce a wide range of valuable compounds. Pulsed Electric Fields (PEF) has been demonstrated to effectively disrupt the microalgae cells and facilitate intracellular extraction. To increase the commercial viability of microalgae, the entire biomass should be exploited with different products extracted and valorized according to the biorefinery scheme. However, demonstrations of multiple component extraction in series are very limited in literature. This study aimed to develop an effective lipid extraction protocol from wet Scenedesmus almeriensis after PEF-treatment with 1.5 MJ·kg
resultsLipid extraction with ethanol:hexane (1:0.41 vol/vol) offered the highest yields from the different protocols tested. PEF-treatment promoted extraction with almost 70% of total lipids extracted against 43% from untreated biomass. An incubation step after PEF-treatment, further improved the yields, up to 83% of total lipids. Increasing the solvent volume by factor 2 offered no improvement. In comparison, extraction with two other systems utilizing only ethanol at room temperature or elevated at 60 °C were ineffective with less than 30% of total lipids extracted. Regarding cascade extraction, carbohydrate release after PEF was detected albeit in low concentrations. PEF-treated samples displayed slightly better kinetics during the enzymatic protein hydrolysis compared to untreated or HPH-treated biomass. The yields from a subsequent lipid extraction were not affected after PEF but were significantly increased for untreated samples (66% of total lipids), while HPH displayed the lowest yields (~ 49% of total lipids).
conclusionsPEF-treatment successfully promoted lipid extraction from S. almeriensis but only in combination with a polar:neutral co-solvent (ethanol:hexane). After enzymatic protein hydrolysis in cascade processing; however, untreated biomass displayed equal lipid yields due to the disruptive effect of the proteolytic enzymes. Therefore, the positive impact of PEF in this scheme is limited on the improved reaction kinetics exhibited during the enzymatic hydrolysis step.
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