ArticleBMC genomics2021
Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature.
Article in BMC genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 45 citations in OpenAlex.
- Crotonylation of ACAT1 K181 contributes to radioresistance by impacting lactate metabolism.Communications biology · 2026Article
- Global Crotonylome Profiling Identifies TaPRXIIB Crotonylation as a Modulator HMolecular plant pathology · 2026Article
- Proteomics: An Essential Tool to Study Plant-Specialized Metabolism.Biomolecules · 2024Review
- GCN5 mediates DNA-PKcs crotonylation for DNA double-strand break repair and determining cancer radiosensitivity.British journal of cancer · 2024Article
- The crotonylated and succinylated proteins of jujube involved in phytoplasma-stress responses.BMC biology · 2024Article
- The ascorbate-glutathione cycle coming of age.Journal of experimental botany · 2024Review
- PlantNh-Kcr: a deep learning model for predicting non-histone crotonylation sites in plants.Plant methods · 2024Article
- Down-regulation of Lon protease 1 lysine crotonylation aggravates mitochondrial dysfunction in polycystic ovary syndrome.MedComm · 2023Article
- Novel insight into functions of ascorbate peroxidase in higher plants: More than a simple antioxidant enzyme.Redox biology · 2023Review
- Global crotonylatome and GWAS revealed aScience advances · 2023Article
- Large-scale analysis of protein crotonylation reveals its diverse functions in Pinellia ternata.BMC plant biology · 2022Article
- Atomically Dispersed Cu Nanozyme with Intensive Ascorbate Peroxidase Mimic Activity Capable of Alleviating ROS-Mediated Oxidation Damage.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Comprehensive Profiling of Paper Mulberry (International journal of molecular sciences · 2022Article
- Qualitative Proteome-Wide Analysis Reveals the Diverse Functions of Lysine Crotonylation inFrontiers in plant science · 2022Article
- Protein lysine crotonylation: past, present, perspective.Cell death & disease · 2021Review
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10 authors at 1 institution in 1 country.
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Abstract
backgroundLow-temperature severely affects the growth and development of chrysanthemum which is one kind of ornamental plant well-known and widely used in the world. Lysine crotonylation is a recently identified post-translational modification (PTM) with multiple cellular functions. However, lysine crotonylation under low-temperature stress has not been studied.
resultsProteome-wide and lysine crotonylation of chrysanthemum at low-temperature was analyzed using TMT (Tandem Mass Tag) labeling, sensitive immuno-precipitation, and high-resolution LC-MS/MS. The results showed that 2017 crotonylation sites were identified in 1199 proteins. Treatment at 4 °C for 24 h and - 4 °C for 4 h resulted in 393 upregulated proteins and 500 downregulated proteins (1.2-fold threshold and P < 0.05). Analysis of biological information showed that lysine crotonylation was involved in photosynthesis, ribosomes, and antioxidant systems. The crotonylated proteins and motifs in chrysanthemum were compared with other plants to obtain orthologous proteins and conserved motifs. To further understand how lysine crotonylation at K136 affected APX (ascorbate peroxidase), we performed a site-directed mutation at K136 in APX. Site-directed crotonylation showed that lysine decrotonylation at K136 reduced APX activity, and lysine complete crotonylation at K136 increased APX activity.
conclusionIn summary, our study comparatively analyzed proteome-wide and crotonylation in chrysanthemum under low-temperature stress and provided insights into the mechanisms of crotonylation in positively regulated APX activity to reduce the oxidative damage caused by low-temperature stress. These data provided an important basis for studying crotonylation to regulate antioxidant enzyme activity in response to low-temperature stress and a new research ideas for chilling-tolerance and freezing-tolerance chrysanthemum molecular breeding.
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