ReviewBiology2021
Microscopy Methods for Biofilm Imaging: Focus on SEM and VP-SEM Pros and Cons.
Review in Biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
100 citing papers in PubMed, 1 synthesis or guideline pooled it, 224 citations in OpenAlex.
- Current therapeutic interventions combating biofilm-related infections in orthopaedics : a systematic review of in vivo animal studies.Bone & joint research · 2022Pooled it
- Evaluating cellular responses of lung and liver cells to graphene oxide functionalized poly(propylene)imine and polyamidoamine dendrimers: insights for biomedical applications.Discover nano · 2026Article
- Design of a Wireless Ultraviolet Germicidal Irradiation System and Validation of Germicidal Potential Against Biofilm-Forming Bacteria and Fungi.Antibiotics (Basel, Switzerland) · 2026Article
- Tackling Biofilm-Forming Pathogens: A Challenge to Overcome in the Fight Against Infectious Diseases.Pathogens (Basel, Switzerland) · 2026Review
- Effects of Adjunctive EDTA and Air-Polishing Powders on Periodontitis-Affected Root Surfaces: A SEM Study.Medicina (Kaunas, Lithuania) · 2026Article
- Detection to Disruption: A Comprehensive Review of Bacterial Biofilms and Therapeutic Advances.Antibiotics (Basel, Switzerland) · 2026Review
- Biofilms in clinical infection: pathophysiology, diagnosis, and the evolving therapeutic landscape.Journal of clinical microbiology · 2026Review
- Povidone iodine demonstrates strong efficacy in reducing Candida biofilm in an in vitro fungal prosthetic infection.Scientific reports · 2026Article
- A nutrient bottleneck controls antibiotic efficacy in structured bacterial populations.Nature communications · 2026Article
- In Vitro Bioactivity of Leaf Extract Fractions and Quercetin-3-International journal of molecular sciences · 2026Article
- Intercalated bacterial biofilms are intrinsic internal components of calcium-based kidney stones.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The Effect of Toothpaste Suspensions on the Formation of Oral Biofilms In Situ.International dental journal · 2026Article
- Flexible Copper-Based TEM Grid for Microscopic Characterization of Aged Magnetotactic Bacteria MS-1 and Their Magnetosome Crystals in Air-Dried Droplet.Molecules (Basel, Switzerland) · 2026Article
- Pathogen Characteristic of Biofilm-Related CAUTI in the Intensive Care Unit.International journal of microbiology · 2026Article
- Synergistic Anti-Biofilm and Bactericidal Activity of Ethanol and Chlorhexidine Combined with EDTA AgainstInfection and drug resistance · 2026Article
- Article
- Effects of Heat-Killed Probiotic Strains on Biofilm Formation, Transcription of Virulence-Associated Genes, and Prevention of UTIs in Mice.Probiotics and antimicrobial proteins · 2025Article
- Inclusion complex of lemongrass essential oil with γ-cyclodextrin: preparation, characterization, and its impact on mango fruit preservation.Food science and biotechnology · 2025Article
- Bioremediation of polycyclic aromatic hydrocarbons contaminated soils/water for environmental remediation.Biodegradation · 2025Review
- Optimizing lipid-lowering synbiotics: strain screening, synergistic consortium formulation, prebiotic modulation, and freeze-drying process engineering for enhanced stability and efficacy.Food chemistry. Molecular sciences · 2025Article
40 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Several imaging methodologies have been used in biofilm studies, contributing to deepening the knowledge on their structure. This review illustrates the most widely used microscopy techniques in biofilm investigations, focusing on traditional and innovative scanning electron microscopy techniques such as scanning electron microscopy (SEM), variable pressure SEM (VP-SEM), environmental SEM (ESEM), and the more recent ambiental SEM (ASEM), ending with the cutting edge Cryo-SEM and focused ion beam SEM (FIB SEM), highlighting the pros and cons of several methods with particular emphasis on conventional SEM and VP-SEM. As each technique has its own advantages and disadvantages, the choice of the most appropriate method must be done carefully, based on the specific aim of the study. The evaluation of the drug effects on biofilm requires imaging methods that show the most detailed ultrastructural features of the biofilm. In this kind of research, the use of scanning electron microscopy with customized protocols such as osmium tetroxide (OsO
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.