Evidence map›Paper›PMID 33443634›Full record

ArticleApplied microbiology and biotechnology2021

Optimization of a T7-RNA polymerase system in Synechococcus sp. PCC 7002 mirrors the protein overproduction phenotype from E. coli BL21(DE3).

Christopher M Jones, Travis C Korosh, David R Nielsen, Brian F Pfleger

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories.Advances in biochemical engineering/biotechnology · 2023
    Article
  3. Review
  4. Regulation ofFrontiers in microbiology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Christopher M JonesChemical Engineering, School for Engineering Matter, Transport, and Energy, Arizona State University, Tempe, AZ, 85287, USA.
Travis C KoroshDepartment of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA.
David R NielsenChemical Engineering, School for Engineering Matter, Transport, and Energy, Arizona State University, Tempe, AZ, 85287, USA.
Brian F PflegerDepartment of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA. pfleger@engr.wisc.edu.ORCID http://orcid.org/0000-0002-9232-9959
Arizona State University · USUniversity of Wisconsin–Madison · US

Funding

BIOTECHNOLOGY TRAINING PROGRAMT32GM008349 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI FOX, BRIAN G · 1989 to 2019
$22.5M
Directorate for Engineering EFRI-1240268NIGMS NIH HHS T32 GM008349
6 · The paper itself

Abstract

With the goal of expanding the diversity of tools available for controlling gene expression in cyanobacteria, the T7-RNA polymerase gene expression system from E. coli BL21(DE3) was adapted and systematically engineered for robust function Synechococcus sp. PCC 7002, a fast-growing saltwater strain. Expression of T7-RNA polymerase was controlled via LacI regulation, while functionality was optimized by both further tuning its expression level along with optimizing the translation initiation region of the expressed gene, in this case an enhanced YFP reporter. Under high CO

Indexed as

SynechococcusDNA-Directed RNA PolymerasesEscherichia coliGene Expression Regulation, BacterialPhenotypeDNA-Directed RNA PolymerasesCyanobacteriaGene circuitsPromotersSynthetic biologyT7-RNA polymerase

Identifiers

PMID33443634
PMCPMC8351450
OpenAlexW3120400863

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.