Evidence map›Paper›PMID 33442944›Full record

ArticleCell proliferation2021

Circadian clock genes promote glioma progression by affecting tumour immune infiltration and tumour cell proliferation.

Zeyu Wang, Guanhua Su, Ziyu Dai, Ming Meng, Hao Zhang, Fan Fan, Zhengzheng Liu, Longbo Zhang, Nathaniel Weygant, Fengqiong He and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
60citing papers in PubMed, 1 pooled it
6.0field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

60 citing papers in PubMed, 1 synthesis or guideline pooled it, 78 citations in OpenAlex.

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  20. Detection of genetic variation in bovineAnimal biotechnology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 2 countries.

Zeyu WangDepartment of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.ORCID https://orcid.org/0000-0002-6675-5399
Guanhua SuDepartment of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.
Ziyu DaiDepartment of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.
Ming MengDepartment of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.
Hao ZhangDepartment of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.
Fan FanDepartment of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.
Zhengzheng LiuDepartment of Oncology, Xiangya Hospital, Central South University, Changsha, China.
Longbo ZhangDepartment of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.
Nathaniel WeygantAcademy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, China.
Fengqiong HeDepartment of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.
Ning FangDepartment of Gastroenterology, The Third Xiangya Hospital, Central South University, Changsha, China.
Liyang ZhangDepartment of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.
Quan ChengDepartment of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.ORCID https://orcid.org/0000-0003-2401-5349
Central South University · CNFujian University of Traditional Chinese Medicine · CN

Funding

China Postdoctoral Science Foundation 2018M63302Health Committee Foundation of Hunan Province C2019186Hunan Provincial Natural Science Foundation of China 2018JJ3838Hunan Provincial Natural Science Foundation of China 2019JJ50963National Nature Science Foundation of China 81402249National Nature Science Foundation of China 81803582National Nature Science Foundation of China 82073893Xiangya Hospital Central South University postdoctoral foundation
6 · The paper itself

Abstract

objectivesCircadian rhythm controls complicated physiological activities in organisms. Circadian clock genes have been related to tumour progression, but its role in glioma is unknown. Therefore, we explored the relationship between dysregulated circadian clock genes and glioma progression. MATERIALS AND

methodsSamples were divided into different groups based on circadian clock gene expression in training dataset (n = 672) and we verified the results in other four validating datasets (n = 1570). The GO and GSEA enrichment analysis were conducted to explore potential mechanism of how circadian clock genes affected glioma progression. The single-cell RNA-Seq analysis was conducted to verified previous results. The immune landscape was evaluated by the ssGSEA and CIBERSORT algorithm. Cell proliferation and viability were confirmed by the CCK8 assay, colony-forming assay and flow cytometry.

resultsThe cluster and risk model based on circadian clock gene expression can predict survival outcome. Samples were scoring by the least absolute shrinkage and selection operator regression analysis, and high scoring tumour was associated with worse survival outcome. Samples in high-risk group manifested higher activation of immune pathway and cell cycle. Tumour immune landscape suggested high-risk tumour infiltrated more immunocytes and more sensitivity to immunotherapy. Interfering TIMELESS expression affected circadian clock gene expression, inhibited tumour cell proliferation and arrested cell cycle at the G0/G1 phase.

conclusionsDysregulated circadian clock gene expression can affect glioma progression by affecting tumour immune landscape and cell cycle. The risk model can predict glioma survival outcome, and this model can also be applied to pan-cancer.

Indexed as

Cell CycleCell ProliferationCircadian ClocksCircadian RhythmDisease ProgressionGliomaHumanscell cyclecircadian clock genesgliomaimmune infiltrationnomogram

Identifiers

PMID33442944
PMCPMC7941241
OpenAlexW3119514529

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.