ArticleScientific reports2021
RNA interference of trehalose-6-phosphate synthase and trehalase genes regulates chitin metabolism in two color morphs of Acyrthosiphon pisum Harris.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 39 citations in OpenAlex.
- RNA Interference TargetingInsects · 2026Article
- Identification, expression profiling, and functional characterization of trehalase genes (OaTRE1 and OaTRE) in Oedaleus asiaticus (Orthoptera: Acridoidea).Journal of insect science (Online) · 2026Article
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- Physiological Response ofInsects · 2025Article
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- Effects of Orally Delivered Double-Stranded RNA ofInsects · 2025Article
- The synthesis, degradation and biological function of trehalose- 6-phosphate.Stress biology · 2025Review
- Enzymes involved in trehalose-chitin synthesis in Haemonchus contortus could be vaccine candidates for goats.Parasites & vectors · 2025Article
- Trehalose 6-phosphate synthase gene rdtps1 contributes to thermal acclimation in Rhyzopertha dominica.BMC genomics · 2024Article
- Trehalase inhibition inJournal of biosciences · 2024Article
- Characterization of Chitin Synthase B Gene (Insects · 2023Article
- Article
- Knockdown of Two Trehalase Genes by RNA Interference Is Lethal to the White-Backed PlanthopperBiomolecules · 2022Article
- Chitinase (CHI) ofFrontiers in physiology · 2022Article
- Effects of abiotic stresses on the expression of chitinase-like genes inFrontiers in physiology · 2022Article
- Article
- Article
- Trehalose and glucose levels regulate feeding behavior of the phloem-feeding insect, the pea aphid Acyrthosiphon pisum Harris.Scientific reports · 2021Article
- Long-Term Effect of Elevated COFrontiers in physiology · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Trehalose-6-phosphate synthase (TPS) and trehalase (TRE) directly regulate trehalose metabolism and indirectly regulate chitin metabolism in insects. Real-time quantitative PCR (RT-qPCR) and RNA interference (RNAi) were used to detect the expressions and functions of the ApTPS and ApTRE genes. Abnormal phenotypes were found after RNAi of ApTRE in the Acyrthosiphon pisum. The molting deformities were observed in two color morphs, while wing deformities were only observed in the red morphs. The RNAi of ApTPS significantly down-regulated the expression of chitin metabolism-related genes, UDP-N-acetyglucosamine pyrophosphorylase (ApUAP), chitin synthase 2 (Apchs-2), Chitinase 2, 5 (ApCht2, 5), endo-beta-N-acetylglucosaminidase (ApENGase) and chitin deacetylase (ApCDA) genes at 24 h and 48 h; The RNAi of ApTRE significantly down-regulated the expression of ApUAP, ApCht1, 2, 8 and ApCDA at 24 h and 48 h, and up-regulated the expression of glucose-6-phosphate isomerase (ApGPI) and Knickkopf protein (ApKNK) genes at 48 h. The RNAi of ApTRE and ApTPS not only altered the expression of chitin metabolism-related genes but also decreased the content of chitin. These results demonstrated that ApTPS and ApTRE can regulate the chitin metabolism, deepen our understanding of the biological functions, and provide a foundation for better understanding the molecular mechanism of insect metamorphosis.
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