Evidence map›Paper›PMID 33441839›Full record

ArticleScientific reports2021

Quantitative immuno-mass spectrometry imaging of skeletal muscle dystrophin.

David P Bishop, Mika T Westerhausen, Florian Barthelemy, Thomas Lockwood, Nerida Cole, Elizabeth M Gibbs, Rachelle H Crosbie, Stanley F Nelson, M Carrie Miceli, Philip A Doble and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 22 citations in OpenAlex.

  1. Enhancing the Sensitivity of Mass Spectrometry Imaging through Spatial Signal Averaging.Journal of the American Society for Mass Spectrometry · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

David P BishopAtomic Medicine Initiative, Faculty of Science, University of Technology Sydney, Ultimo, NSW, Australia.
Mika T WesterhausenAtomic Medicine Initiative, Faculty of Science, University of Technology Sydney, Ultimo, NSW, Australia.
Florian BarthelemyCenter for Duchenne Muscular Dystrophy, University of California, Los Angeles, Los Angeles, CA, USA.
Thomas LockwoodAtomic Medicine Initiative, Faculty of Science, University of Technology Sydney, Ultimo, NSW, Australia.
Nerida ColeARC Training Centre in Biodevices, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, VIC, Australia.
Elizabeth M GibbsCenter for Duchenne Muscular Dystrophy, University of California, Los Angeles, Los Angeles, CA, USA.
Rachelle H CrosbieCenter for Duchenne Muscular Dystrophy, University of California, Los Angeles, Los Angeles, CA, USA.
Stanley F NelsonCenter for Duchenne Muscular Dystrophy, University of California, Los Angeles, Los Angeles, CA, USA.
M Carrie MiceliCenter for Duchenne Muscular Dystrophy, University of California, Los Angeles, Los Angeles, CA, USA.
Philip A DobleAtomic Medicine Initiative, Faculty of Science, University of Technology Sydney, Ultimo, NSW, Australia.
Jonathan WanagatVeterans Administration Greater Los Angeles Healthcare System, Los Angeles, USA. jwanagat@mednet.ucla.edu.
University of California, Los Angeles · USUniversity of Technology Sydney · AUSwinburne University of Technology · AUVA Greater Los Angeles Healthcare System · US

Funding

Structure-Function Analysis of SarcospanR01AR048179 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CROSBIE, RACHELLE HOPE · 2001 to 2023
$6.9M
Muscle Cell Biology, Pathophysiology, and TherapeuticsT32AR065972 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Rachelle Hope Crosbie, THOMAS A. RANDO · 2016 to 2026
$3.9M
Mitochondrial biogenesis, genetics and cell loss in mammalian agingR01AG055518 · NIA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AIKEN, JUDD M., HERBST, ALLEN · 2017 to 2022
$2.0M
Restoration of muscle cell adhesion to treat cardiomyopathy in muscular dystrophyR01HL126204 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CROSBIE, RACHELLE HOPE · 2016 to 2019
$1.5M
Mitochondrial biogenesis, genetics and cell loss in mammalian agingK02AG059847 · NIA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WANAGAT, JONATHAN · 2018 to 2022
$801k
Mitochondrial DNA Deletion Mutations: Biomarkers of AgingR56AG060880 · NIA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AIKEN, JUDD M., ALLISON, DAVID B · 2018 to 2018
$574k
Quantitative imaging of the dystrophin-glycoprotein complexR21AR072950 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DOBLE, PHILIP, WANAGAT, JONATHAN · 2019 to 2020
$357k
NHLBI NIH HHS R01 HL126204NIAMS NIH HHS R01 AR048179NIAMS NIH HHS R21 AR072950NIAMS NIH HHS T32 AR065972NIA NIH HHS K02 AG059847NIA NIH HHS R01 AG055518NIA NIH HHS R56 AG060880
6 · The paper itself

Abstract

Emerging and promising therapeutic interventions for Duchenne muscular dystrophy (DMD) are confounded by the challenges of quantifying dystrophin. Current approaches have poor precision, require large amounts of tissue, and are difficult to standardize. This paper presents an immuno-mass spectrometry imaging method using gadolinium (Gd)-labeled anti-dystrophin antibodies and laser ablation-inductively coupled plasma-mass spectrometry to simultaneously quantify and localize dystrophin in muscle sections. Gd is quantified as a proxy for the relative expression of dystrophin and was validated in murine and human skeletal muscle sections following k-means clustering segmentation, before application to DMD patients with different gene mutations where dystrophin expression was measured up to 100 µg kg

Indexed as

AdolescentAged, 80 and overAnimalsChildDystrophinFemaleFluorescent Antibody TechniqueGadoliniumHumansImmunohistochemistryMaleMass SpectrometryMiceMuscle Fibers, SkeletalMuscular Dystrophy, DuchenneMutationDystrophinGadolinium

Identifiers

PMID33441839
PMCPMC7806610
OpenAlexW3118534846

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.