Evidence map›Paper›PMID 33441763›Full record

ArticleScientific reports2021

Immune modulation resulting from MR-guided high intensity focused ultrasound in a model of murine breast cancer.

Brett Z Fite, James Wang, Aris J Kare, Asaf Ilovitsh, Michael Chavez, Tali Ilovitsh, Nisi Zhang, Weiyu Chen, Elise Robinson, Hua Zhang and 9 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.

0numbers the graph read from it
0cells of the map it votes in
54citing papers in PubMed
6.3field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

54 citing papers in PubMed, 84 citations in OpenAlex.

  1. Trial
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  11. Sex parity in stimulation of murine lymphocyte trafficking in response to fever-range systemic thermal therapy.International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group · 2025
    Article
  12. Article
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  15. Overview of Therapeutic Ultrasound Applications and Safety Considerations: 2024 Update.Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine · 2025
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 2 institutions in 1 country.

Brett Z FiteDepartment of Radiology, Stanford University, 3165 Porter Dr, Palo Alto, CA, 94305, USA.
James WangDepartment of Radiology, Stanford University, 3165 Porter Dr, Palo Alto, CA, 94305, USA.
Aris J KareDepartment of Radiology, Stanford University, 3165 Porter Dr, Palo Alto, CA, 94305, USA.
Asaf IlovitshDepartment of Radiology, Stanford University, 3165 Porter Dr, Palo Alto, CA, 94305, USA.
Michael ChavezDepartment of Biomedical Engineering, Stanford University, Palo Alto, CA, 94305, USA.
Tali IlovitshDepartment of Radiology, Stanford University, 3165 Porter Dr, Palo Alto, CA, 94305, USA.
Nisi ZhangDepartment of Radiology, Stanford University, 3165 Porter Dr, Palo Alto, CA, 94305, USA.
Weiyu ChenDepartment of Radiology, Stanford University, 3165 Porter Dr, Palo Alto, CA, 94305, USA.
Elise RobinsonDepartment of Radiology, Stanford University, 3165 Porter Dr, Palo Alto, CA, 94305, USA.
Hua ZhangDepartment of Biomedical Engineering, University of California Davis, Davis, CA, 95616, USA.
Azadeh KheirolomoomDepartment of Radiology, Stanford University, 3165 Porter Dr, Palo Alto, CA, 94305, USA.
Matthew T SilvestriniDepartment of Biomedical Engineering, University of California Davis, Davis, CA, 95616, USA.
Elizabeth S InghamDepartment of Biomedical Engineering, University of California Davis, Davis, CA, 95616, USA.
Lisa M MahakianDepartment of Biomedical Engineering, University of California Davis, Davis, CA, 95616, USA.
Sarah M TamDepartment of Biomedical Engineering, University of California Davis, Davis, CA, 95616, USA.
Ryan R DavisDepartment of Pathology and Laboratory Medicine, School of Medicine, University of California Davis, Sacramento, CA, 95817, USA.
Clifford G TepperDepartment of Biochemistry and Molecular Medicine, School of Medicine, University of California Davis, Sacramento, CA, 95817, USA.
Alexander D BorowskyDepartment of Pathology and Laboratory Medicine, School of Medicine, University of California Davis, Sacramento, CA, 95817, USA.
Katherine W FerraraDepartment of Radiology, Stanford University, 3165 Porter Dr, Palo Alto, CA, 94305, USA. kwferrar@stanford.edu.
Stanford University · USUniversity of California, Davis · US

Funding

Staff InvestigatorsP30CA093373 · NCI · UNIVERSITY OF CALIFORNIA DAVIS · PI Laura Marcu · 2002 to 2026
$84.9M
Supplement to Enhance Wellness and Resiliency in the Graduate EnvironmentT32GM007276 · NIGMS · STANFORD UNIVERSITY · PI MORRISON, ASHBY J. · 1985 to 2023
$32.9M
Image-guided ultrasound therapy and drug delivery in pancreatic cancerR01CA210553 · NCI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI FERRARA, KATHERINE W · 2016 to 2020
$3.2M
HIFU-immunotherapy in pancreatic cancerR01CA253316 · NCI · STANFORD UNIVERSITY · PI FERRARA, KATHERINE W · 2020 to 2024
$3.1M
NCI NIH HHS P30 CA093373NCI NIH HHS R01 CA210553NCI NIH HHS R01 CA253316NIGMS NIH HHS T32 GM007276NIH HHS R01CA210553
6 · The paper itself

Abstract

High intensity focused ultrasound (HIFU) rapidly and non-invasively destroys tumor tissue. Here, we sought to assess the immunomodulatory effects of MR-guided HIFU and its combination with the innate immune agonist CpG and checkpoint inhibitor anti-PD-1. Mice with multi-focal breast cancer underwent ablation with a parameter set designed to achieve mechanical disruption with minimal thermal dose or a protocol in which tumor temperature reached 65 °C. Mice received either HIFU alone or were primed with the toll-like receptor 9 agonist CpG and the checkpoint modulator anti-PD-1. Both mechanical HIFU and thermal ablation induced a potent inflammatory response with increased expression of Nlrp3, Jun, Mefv, Il6 and Il1β and alterations in macrophage polarization compared to control. Furthermore, HIFU upregulated multiple innate immune receptors and immune pathways, including Nod1, Nlrp3, Aim2, Ctsb, Tlr1/2/4/7/8/9, Oas2, and RhoA. The inflammatory response was largely sterile and consistent with wound-healing. Priming with CpG attenuated Il6 and Nlrp3 expression, further upregulated expression of Nod2, Oas2, RhoA, Pycard, Tlr1/2 and Il12, and enhanced T-cell number and activation while polarizing macrophages to an anti-tumor phenotype. The tumor-specific antigen, cytokines and cell debris liberated by HIFU enhance response to innate immune agonists.

Indexed as

AnimalsBreast NeoplasmsDisease Models, AnimalHigh-Intensity Focused Ultrasound AblationHumansImmunityMagnetic Resonance SpectroscopyMiceMice, Inbred StrainsNeoplasmsOligodeoxyribonucleotidesProgrammed Cell Death 1 ReceptorPyrinUltrasonographyCpG ODN 1826Mefv protein, mouseOligodeoxyribonucleotidesProgrammed Cell Death 1 ReceptorPyrin

Identifiers

PMID33441763
PMCPMC7806949
OpenAlexW3118527364

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.