ArticleParasites & vectors2021
Spatial distance between sites of sampling associated with genetic variation among Neospora caninum in aborted bovine foetuses from northern Italy.
Article in Parasites & vectors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 2 of them syntheses that pooled it.
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Who cites it
13 citing papers in PubMed, 2 syntheses or guidelines pooled it, 13 citations in OpenAlex.
- Bovine infectious abortion: a systematic review and meta-analysis.Frontiers in veterinary science · 2023Pooled it
- Neospora caninum infection in aborting bovines and lost fetuses: A systematic review and meta-analysis.PloS one · 2022Pooled it
- Review
- Infectious causes of pregnancy loss in cattle: a review of loss intensity and major reproductive pathogens involved.Animal reproduction · 2026Review
- Genetic Diversity of Neospora Caninum in Palestinian Cattle and Sheep: Insights From Nc-5 Gene Detection and MS10 Microsatellite Analysis.Veterinary medicine and science · 2026Article
- Molecular investigation of Neospora caninum in cattle in the Khomas region of Namibia.The Onderstepoort journal of veterinary research · 2025Article
- Serological Prevalence ofAnimals : an open access journal from MDPI · 2023Article
- The Utility of Serological Analysis forAnimals : an open access journal from MDPI · 2022Article
- Prevalence of Neospora caninum antibodies in fattening pigs and sows from intensive farms in northern Italy.Parasitology research · 2022Article
- Detection of Anti-Veterinary sciences · 2022Article
- Seroprevalence and risk factors ofFrontiers in veterinary science · 2022Article
- A holstein heifer infected withFrontiers in veterinary science · 2022Article
- Molecular Survey onMicroorganisms · 2021Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundNeospora caninum, a coccidian protozoan, represents an important cause of bovine abortion. Available N. caninum strains show considerable variation in vitro and in vivo, including different virulence in cattle. To which extent sexual recombination, which is possible in the intestines of domestic dogs and closely related carnivores as definitive hosts, contributes to this variation is not clear yet.
methodsAborted bovine foetuses were collected between 2015 and early 2019 from Italian Holstein Friesian dairy herds suffering from reproductive problems. A total of 198 samples were collected from 165 intensive farms located in Lombardy, northern Italy. N. caninum samples were subjected to multilocus-microsatellite genotyping using ten previously established microsatellite markers. In addition to our own data, those from a recent study providing data on five markers from other northern Italian regions were included and analysed.
resultsOf the 55 samples finally subjected to genotyping, 35 were typed at all or 9 out of 10 loci and their individual multilocus-microsatellite genotype (MLMG) determined. Linear regression revealed a statistically significant association between the spatial distance of the sampling sites with the genetic distance of N. caninum MLMGs (P < 0.001). Including data from this and a previous North Italian study into eBURST analysis revealed that several of N. caninum MLMGs from northern Italy separate into four groups; most of the samples from Lombardy clustered in one of these groups. Principle component analysis revealed similar clusters and confirmed MLMG groups identified by eBURST. Variations observed between MLMGs were not equally distributed over all loci, but predominantly observed in MS7, MS6A, or MS10.
conclusionsOur findings confirm the concept of local N. caninum subpopulations. The geographic distance of sampling was associated with the genetic distance as determined by microsatellite typing. Results suggest that multi-parental recombination in N. caninum is a rare event, but does not exclude uniparental mating. More comprehensive studies on microsatellites in N. caninum and related species like Toxoplasma gondii should be undertaken, not only to improve genotyping capabilities, but also to understand possible functions of these regions in the genomes of these parasites.
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