ArticleMalaria journal2021
Culturable bacteria associated with Anopheles darlingi and their paratransgenesis potential.
Article in Malaria journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Evaluating paratransgenesis using engineered symbiotic bacteria for Plasmodium inhibition in mosquito vectors: A systematic review.PLoS neglected tropical diseases · 2026Pooled it
- Leveraging microbial ecology for mosquito-borne disease control.Trends in parasitology · 2025Review
- Article
- Metagenomic analysis evidences a core virome in Anopheles darlingi from three contrasting Colombian ecoregions.PloS one · 2025Article
- Isolation and characterization of the midgut microbiota of Aedes albopictus to identify suitable candidates for paratransgenesis.European journal of public health · 2025Article
- The Effect of Larval Exposure to Heavy Metals on the Gut Microbiota Composition of AdultTropical medicine and infectious disease · 2024Article
- Fungi from Anopheles darlingi Root, 1926, larval breeding sites in the Brazilian Amazon.PloS one · 2024Article
- Biotechnological Potential of Microorganisms for Mosquito Population Control and Reduction in Vector Competence.Insects · 2023Review
- Comparative assessment of the bacterial communities associated with Anopheles darlingi immature stages and their breeding sites in the Brazilian Amazon.Parasites & vectors · 2023Article
- Bacterial Microbiota from Lab-Reared and Field-CapturedMicroorganisms · 2023Article
- Egg-laying by female Aedes aegypti shapes the bacterial communities of breeding sites.BMC biology · 2023Article
- Bacterial Community Diversity and Bacterial Interaction Network in Eight Mosquito Species.Genes · 2022Article
- Whole Genome Sequencing and CRISPR/Cas9 Gene Editing of EnterotoxigenicInternational journal of molecular sciences · 2022Article
- Malaria-Transmitting Vectors Microbiota: Overview and Interactions WithFrontiers in microbiology · 2022Review
- Paratransgenic manipulation of a tsetse microRNA alters the physiological homeostasis of the fly's midgut environment.PLoS pathogens · 2021Article
- Correction to: Culturable bacteria associated with Anopheles darlingi and their paratransgenesis potential.Malaria journal · 2021Article
- Multi-Omic Analysis of Symbiotic Bacteria Associated WithFrontiers in microbiology · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
16 authors at 6 institutions in 2 countries.
Funding
Abstract
backgroundMalaria remains a major public health problem in South America, mostly in the Amazon region. Among newly proposed ways of controlling malaria transmission to humans, paratransgenesis is a promising alternative. Paratransgenesis aims to inhibit the development of parasites within the vector through the action of genetically modified bacteria. The first step towards successful paratransgenesis in the Amazon is the identification of Anopheles darlingi symbiotic bacteria, which are transmitted vertically among mosquitoes, and are not pathogenic to humans.
methodsCulturable bacteria associated with An. darlingi and their breeding sites were isolated by conventional microbiological techniques. Isolated strains were transformed with a GFP expressing plasmid, pSPT-1-GFP, and reintroduced in mosquitoes by feeding. Their survival and persistence in the next generation was assessed by the isolation of fluorescent bacteria from eggs, larvae, pupae and adult homogenates.
resultsA total of 179 bacterial strains were isolated from samples from two locations, Coari and Manaus. The predominant genera identified in this study were Acinetobacter, Enterobacter, Klebsiella, Serratia, Bacillus, Elizabethkingia, Stenotrophomonas and Pantoea. Two isolated strains, Serratia-Adu40 and Pantoea-Ovo3, were successfully transformed with the pSPT-1-GFP plasmid and expressed GFP. The fluorescent bacteria fed to adult females were transferred to their eggs, which persisted in larvae and throughout metamorphosis, and were detected in adult mosquitoes of the next generation.
conclusionSerratia-Adu40 and Pantoea-Ovo3 are promising candidates for paratransgenesis in An. darlingi. Further research is needed to determine if these bacteria are vertically transferred in nature.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.