Evidence map›Paper›PMID 33440839›Full record

ArticleInternational journal of molecular sciences2021

Effects of Survival Motor Neuron Protein on Germ Cell Development in Mouse and Human.

Wei-Fang Chang, Min Peng, Jing Hsu, Jie Xu, Huan-Chieh Cho, Hsiu-Mei Hsieh-Li, Ji-Long Liu, Chung-Hao Lu, Li-Ying Sung

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 4 countries.

Wei-Fang ChangInstitute of Biotechnology, National Taiwan University, Taipei 106, Taiwan.ORCID 0000-0001-8754-5991
Min PengInstitute of Biotechnology, National Taiwan University, Taipei 106, Taiwan.
Jing HsuInstitute of Biotechnology, National Taiwan University, Taipei 106, Taiwan.
Jie XuCenter for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center, Ann Arbor, MI 48109, USA.ORCID 0000-0001-9452-2878
Huan-Chieh ChoAnimal Resource Center, National Taiwan University, Taipei 106, Taiwan.
Hsiu-Mei Hsieh-LiDepartment of Life Science, National Taiwan Normal University, Taipei 116, Taiwan.
Ji-Long LiuMRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3PT, UK.
Chung-Hao LuDepartment of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei 105, Taiwan.ORCID 0000-0001-5588-4139
Li-Ying SungInstitute of Biotechnology, National Taiwan University, Taipei 106, Taiwan.ORCID 0000-0003-1221-2476
National Taiwan University · TWMackay Memorial Hospital · TWNational Taiwan Normal University · TWShanghaiTech University · CNUniversity of Michigan · US

Funding

Medical Research Council MC_U137788471Medical Research Council MC_UU_12021/3Ministry of Science and Technology, Taiwan Grant Number 106-2313-B-002-039-MY3 and 109-2313-B-002 -003 -MY2 to L. S., 106-2314-B-195-006-MY3 and 109-2314-B-195-011 to C. L. and 108-2811-B-002-526 and 109-2811-B-002-544 to W. C.
6 · The paper itself

Abstract

Survival motor neuron (SMN) is ubiquitously expressed in many cell types and its encoding gene, survival motor neuron 1 gene (SMN1), is highly conserved in various species. SMN is involved in the assembly of RNA spliceosomes, which are important for pre-mRNA splicing. A severe neurogenic disease, spinal muscular atrophy (SMA), is caused by the loss or mutation of SMN1 that specifically occurred in humans. We previously reported that SMN plays roles in stem cell biology in addition to its roles in neuron development. In this study, we investigated whether SMN can improve the propagation of spermatogonia stem cells (SSCs) and facilitate the spermatogenesis process. In in vitro culture, SSCs obtained from SMA model mice showed decreased growth rate accompanied by significantly reduced expression of spermatogonia marker promyelocytic leukemia zinc finger (PLZF) compared to those from heterozygous and wild-type littermates; whereas SMN overexpressed SSCs showed enhanced cell proliferation and improved potency. In vivo, the superior ability of homing and complete performance in differentiating progeny was shown in SMN overexpressed SSCs in host seminiferous tubule of transplant experiments compared to control groups. To gain insights into the roles of SMN in clinical infertility, we derived human induced pluripotent stem cells (hiPSCs) from azoospermia patients (AZ-hiPSCs) and from healthy control (ct-hiPSCs). Despite the otherwise comparable levels of hallmark iPCS markers, lower expression level of

Indexed as

Cell DifferentiationAnimalsAzoospermiaCells, CulturedCell Self RenewalCell SurvivalDisease Models, AnimalGene ExpressionGerm CellsHumansInduced Pluripotent Stem CellsKruppel-Like Factor 4MaleMiceMotor NeuronsSpermatogoniaKLF4 protein, humanKlf4 protein, mouseKruppel-Like Factor 4Survival of Motor Neuron 1 Proteinazoospermiaspermatogenesissurvival motor neuron

Identifiers

PMID33440839
PMCPMC7827477
OpenAlexW3118734710

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.