Evidence map›Paper›PMID 33439202›Full record

ArticleLab on a chip2021

Contact photolithography-free integration of patterned and semi-transparent indium tin oxide stimulation electrodes into polydimethylsiloxane-based heart-on-a-chip devices for streamlining physiological recordings.

Joycelyn K Yip, Debarghya Sarkar, Andrew P Petersen, Jennifer N Gipson, Jun Tao, Salil Kale, Megan L Rexius-Hall, Nathan Cho, Natalie N Khalil, Rehan Kapadia and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Lab on a chip, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.7field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 2 countries.

Joycelyn K YipLaboratory for Living Systems Engineering, Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA. mlmccain@usc.edu.
Debarghya SarkarMing Hsieh Department of Electrical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA. rkapadia@usc.edu.
Andrew P PetersenLaboratory for Living Systems Engineering, Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA. mlmccain@usc.edu.
Jennifer N GipsonLaboratory for Living Systems Engineering, Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA. mlmccain@usc.edu.
Jun TaoMing Hsieh Department of Electrical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA. rkapadia@usc.edu.
Salil KaleMing Hsieh Department of Electrical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA. rkapadia@usc.edu.
Megan L Rexius-HallLaboratory for Living Systems Engineering, Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA. mlmccain@usc.edu.
Nathan ChoLaboratory for Living Systems Engineering, Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA. mlmccain@usc.edu.
Natalie N KhalilLaboratory for Living Systems Engineering, Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA. mlmccain@usc.edu.
Rehan KapadiaMing Hsieh Department of Electrical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA. rkapadia@usc.edu.
Megan L McCainLaboratory for Living Systems Engineering, Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA. mlmccain@usc.edu and Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of USC, University of Southern California, Los Angeles, CA 90033, USA.ORCID 0000-0003-1908-6783
University of Southern California · US

Funding

Engineering a Microphysiological System to Model the Infarct Border Zone and Interrogate Oxygen-Dependent Cell-Cell Communication in the MyocardiumR01HL153286 · NHLBI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Megan Laura McCain · 2020 to 2026
$3.4M
American Heart Association-American Stroke Association 0815729DAmerican Heart Association-American Stroke Association 16SDG29950005NHLBI NIH HHS R01 HL153286
6 · The paper itself

Abstract

Controlled electrical stimulation is essential for evaluating the physiology of cardiac tissues engineered in heart-on-a-chip devices. However, existing stimulation techniques, such as external platinum electrodes or opaque microelectrode arrays patterned on glass substrates, have limited throughput, reproducibility, or compatibility with other desirable features of heart-on-a-chip systems, such as the use of tunable culture substrates, imaging accessibility, or enclosure in a microfluidic device. In this study, indium tin oxide (ITO), a conductive, semi-transparent, and biocompatible material, was deposited onto glass and polydimethylsiloxane (PDMS)-coated coverslips as parallel or point stimulation electrodes using laser-cut tape masks. ITO caused substrate discoloration but did not prevent brightfield imaging. ITO-patterned substrates were microcontact printed with arrayed lines of fibronectin and seeded with neonatal rat ventricular myocytes, which assembled into aligned cardiac tissues. ITO deposited as parallel or point electrodes was connected to an external stimulator and used to successfully stimulate micropatterned cardiac tissues to generate calcium transients or propagating calcium waves, respectively. ITO electrodes were also integrated into the cantilever-based muscular thin film (MTF) assay to stimulate and quantify the contraction of micropatterned cardiac tissues. To demonstrate the potential for multiple ITO electrodes to be integrated into larger, multiplexed systems, two sets of ITO electrodes were deposited onto a single substrate and used to stimulate the contraction of distinct micropatterned cardiac tissues independently. Collectively, these approaches for integrating ITO electrodes into heart-on-a-chip devices are relatively facile, modular, and scalable and could have diverse applications in microphysiological systems of excitable tissues.

Indexed as

Lab-On-A-Chip DevicesTin CompoundsAnimalsDimethylpolysiloxanesRatsReproducibility of ResultsDimethylpolysiloxanesindium tin oxideTin Compounds

Identifiers

PMID33439202
PMCPMC7968549
OpenAlexW3120576634

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.