Evidence map›Paper›PMID 33438816›Full record

ArticleThe Kaohsiung journal of medical sciences2021

Level of Foxp3, DNMTs, methylation of Foxp3 promoter region, and CD4 + CD25 + CD127low regulatory T cells in vulvar lichen sclerosus.

Lin Wang, Jin-Ling Yi, Hai-Yan Chen, Pei-Liang Wang, Yan-Li Shen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Kaohsiung journal of medical sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 2 pooled it
1.3field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 2 syntheses or guidelines pooled it, 9 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Trial
  4. Article
  5. Review
  6. Lichen sclerosus: The 2023 update.Frontiers in medicine · 2023
    Review
  7. Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Lin WangORCID https://orcid.org/0000-0002-0387-346X
Jin-Ling YiDepartment of Gynecology, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
Hai-Yan ChenDepartment of Gynecology, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
Pei-Liang WangDepartment of Gynecology, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
Yan-Li ShenDepartment of Gynecology, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
Xinjiang Medical University · CN

Funding

Natural Science Foundation of Xinjiang Uygur Autonomous Region 2016D01C236
6 · The paper itself

Abstract

This study is to investigate the pathogenesis of vulvar lichen sclerosus (VLS) by analyzing the level of Foxp3, DNMTs, methylation of Foxp3 promoter region, and CD4 + CD25 + CD127low Regulatory T cells (Tregs). This study enrolled 15 VLS patients and 25 controls. Lesional and extralesional vulvar skin tissues, normal vulvar skin tissues and peripheral blood were collected. Compared with the control group, Foxp3 protein in the lesional and extralesional skin of VLS group was significantly reduced. The levels of DNMT1 and DNMT3b proteins in lesional skin of VLS group were significantly increased. There was no difference in the total methylation rates of the promoter region of the Foxp3 gene. The methylation rates of CpG1, CpG4, CpG9, and CpG10 were significantly higher in lesional skin of VLS group than in control group. There was no correlation between the total methylation rates of 10 CpG sites and the level of Foxp3 and DNMT1 proteins; there was a positive correlation between Foxp3 and DNMT1 protein in lesional skin of VLS group (r = 0.675, p < 0.05), and a negative correlation (r = -0.665, p < 0.05) in extralesional skin of VLS group. However, there was no correlation of Foxp3 with DNMT3b. The number of CD4 + CD25 + CD127low Tregs VLS decreased significantly. The expression of Foxp3 protein and the quantity of CD4 + CD25 + CD127low Tregs in patients with VLS decreased, which may cause local or systemic abnormal immunosuppression of Tregs, leading to the occurrence of VLS. This may be related with methylation or DNMT1, which needs further verification.

Indexed as

Promoter Regions, GeneticAdultCD4-Positive T-LymphocytesCpG IslandsDNA (Cytosine-5-)-Methyltransferase 1DNA MethylationDown-RegulationFemaleForkhead Transcription FactorsHumansImmune ToleranceImmunosuppression TherapyInterleukin-2 Receptor alpha SubunitInterleukin-7 Receptor alpha SubunitMethylationMiddle AgedDNA (Cytosine-5-)-Methyltransferase 1DNMT1 protein, humanForkhead Transcription FactorsFOXP3 protein, humanIL2RA protein, humanIL7R protein, humanInterleukin-2 Receptor alpha SubunitInterleukin-7 Receptor alpha SubunitCD127CD4 + CD25+ TregFoxp3methylationvulvar lichen sclerosus

Identifiers

PMID33438816
PMCPMC11896417
OpenAlexW3120098762

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.