Evidence map›Paper›PMID 33437849›Full record

ArticleBio-protocol2014

Pulse Chase of Suspension Cells.

Lai-Yee Wong, QiMing Liang, Kevin Brulois, Jae Jung

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Lai-Yee WongDepartment of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA, USA.
QiMing LiangDepartment of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA, USA.
Kevin BruloisDepartment of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA, USA.
Jae JungDepartment of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA, USA.

Funding

Roles of the K3 and K5 in KSHV Persistence and PathogenesisP01CA180779 · NCI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI JUNG, JAE U · 2013 to 2017
$7.3M
Molecular Basis of KSHV PathogenesisR01CA082057 · NCI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI JUNG, JAE U · 1999 to 2016
$5.1M
MOLECULAR BASIS FOR HERPESVIRUS SAIMIRI ONCOGENICITYR01CA031363 · NCI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI JUNG, JAE U · 1985 to 2014
$4.9M
Role of viral BCI-2 mediated anti-apoptosis and-autophagyR01AI073099 · NIAID · UNIVERSITY OF SOUTHERN CALIFORNIA · PI JUNG, JAE U · 2007 to 2017
$4.3M
KSHV RTA: more than viral replicationR01CA115284 · NCI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI JUNG, JAE U · 2005 to 2015
$3.1M
NCI NIH HHS P01 CA180779NCI NIH HHS R01 CA031363NCI NIH HHS R01 CA082057NCI NIH HHS R01 CA115284NIAID NIH HHS R01 AI073099
6 · The paper itself

Abstract

Pulse-chase method is a powerful technique used to follow the dynamics of proteins over a period of time. The expression level, processing, transport, secretion or half-life of proteins can be tracked by metabolically labeling the cells, such as with radiolabeled amino acids (pulse step). This protocol describes the condition used to study the folding and disulfide bond formation of immunoglobulin in suspension cells. With some minor modifications, this protocol can be adapted to study the degradation rate or the secretion of target proteins.

Identifiers

PMID33437849
PMCPMC7799445

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.