ArticleJournal of orthopaedic translation2021
Molecular mechanisms of osteogenesis and antibacterial activity of Cu-bearing Ti alloy in a bone defect model with infection
Article in Journal of orthopaedic translation, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Cellular mechanisms of osteoporosis: A comprehensive perspective on ferroptosis, cuproptosis and lipid metabolism abnormalities.Biomaterials translational · 2026Review
- Unlocking bone repair in osteoporosis by targeting the angiogenic niche.Frontiers in endocrinology · 2026Review
- Multilayer Ti-Cu Oxide Coatings on Ti6Al4V: Balancing Antibacterial Activity, Mechanical Strength, Corrosion Resistance, and Cytocompatibility.Journal of functional biomaterials · 2025Article
- Enhancing the Antibacterial and Biointegrative Properties of Microporous Titanium Surfaces Using Various Metal Coatings: A Comparative Study.Prosthesis (Basel, Switzerland) · 2025Article
- Strategic incorporation of metal ions in bone regenerative scaffolds: multifunctional platforms for advancing osteogenesis.Regenerative biomaterials · 2025Review
- Research progress of implantable materials in antibacterial treatment of bone infection.Frontiers in bioengineering and biotechnology · 2025Review
- Surface-modified titanium and titanium-based alloys for improved osteogenesis: A critical review.Heliyon · 2024Review
- Research progress on antibacterial activity of medical titanium alloy implant materials.Odontology · 2023Review
- Transformation of arginine into zero-dimensional nanomaterial endows the material with antibacterial and osteoinductive activity.Science advances · 2023Article
- 3D-printed NIR-responsive shape memory polyurethane/magnesium scaffolds with tight-contact for robust bone regeneration.Bioactive materials · 2022Article
- Article
- Anti-infection mechanism of a novel dental implant made of titanium-copper (TiCu) alloy and its mechanism associated with oral microbiology.Bioactive materials · 2022Article
- Promoting osteointegration effect of Cu-alloyed titanium in ovariectomized rats.Regenerative biomaterials · 2022Article
- Multifunctional inorganic biomaterials: New weapons targeting osteosarcoma.Frontiers in molecular biosciences · 2022Review
- Antibiotics- and Heavy Metals-Based Titanium Alloy Surface Modifications for Local Prosthetic Joint Infections.Antibiotics (Basel, Switzerland) · 2021Review
- Copper-based biomaterials for bone and cartilage tissue engineering.Journal of orthopaedic translation · 2021Review
- Editorial: Fresh perspectives on established ideas.Journal of orthopaedic translation · 2021Article
- Recent Advances in Research on Antibacterial Metals and Alloys as Implant Materials.Frontiers in cellular and infection microbiology · 2021Review
- Meta-analysis of proteomics data from osteoblasts, bone, and blood: Insights into druggable targets, active factors, and potential biomarkers for bone biomaterial design.Journal of tissue engineeringArticle
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe antibacterial activity of copper (Cu)-alloy biomaterials has shown a great potential in clinical application. Here, we evaluated the osteogenesis and antibacterial effects of Ti6Al4V-6.5wt%Cu alloy in an in vivo model of infected bone defects and determine their responsible proteins and pathways using proteomics.
methodsAfter bone defects were filled with Ti6Al4V and Ti6Al4V-6.5wt%Cu implants for 6 week, the tissue and bone samples around the implants were harvested for radiographic, micro-CT, histological, and bone-related gene expression analyses. An iTRAQ-based protein identification/quantification approach was used to analyze the osteogenic and antibacterial effects of Ti6Al4V-6.5wt%Cu alloy.
resultsImaging and histological results showed Ti6Al4V alloy induced a stronger inflammatory response than Ti6Al4V-6.5wt%Cu alloy; imaging results and osteogenic protein levels showed Ti6Al4V-6.5wt%Cu alloy exerted a stronger osteogenic effect. In vitro experiment, we found the Ti6Al4V-6.5wt%Cu had significant antibacterial effects and inhibited the activity of Staphylococcus aureus in the early stage. In addition, the bacterial biofilm formed in Ti6Al4V-6.5wt%Cu group was significantly lower than that in Ti6Al4V group. Proteomic screening of 4279 proteins resulted in 35 differentially expressed proteins for further examination which were mainly associated with the cellular process, metabolic process, stimulus response, and cellular component organization. In further exploration of the mechanism of osteogenic mineralization of Ti6Al4V-6.5wt%Cu alloy, we found out SDC4 and AGRN were the top two target proteins associated with osteogenic differentiation and bone mineralization.
conclusionTi6Al4V-6.5wt%Cu alloy shows a great potential as a bone implant material due to its positive effects against bacterial infection and on bone formation. THE TRANSLATIONAL POTENTIAL OF THIS ARTICLE: At present, titanium alloys and other non-antibacterial metal materials are used in orthopedic internal fixation operations. Our study demonstrates that Ti6Al4V-6.5wt%Cu alloy has good antibacterial and osteogenic effects in vivo and in vitro. This means that Ti6Al4V-6.5wt%Cu alloy may become a new kind of antimicrobial metallic material as internal fixation material to continuously exert its antimicrobial effects and reduce the infection rate after clinical internal fixation.
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