ArticleJournal of orthopaedic translation2021
One strike loading organ culture model to investigate the post-traumatic disc degenerative condition.
Article in Journal of orthopaedic translation, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 38 citations in OpenAlex.
- Research advances in microneedle-exosome delivery systems for the treatment of multisystem diseases.Regenerative therapy · 2026Review
- Harnessing piezoelectric stimulation to modulate PI3K-AKT signaling for intervertebral disc regeneration.Bioactive materials · 2026Article
- Region-dependent expression and function of integrin α5β1 in protecting against disc degenerationFrontiers in bioengineering and biotechnology · 2026Article
- The Biomechanical Landscape of Lumbar Disc Herniation: Mechanobiological Insights Into Injury and Regeneration.Neurospine · 2026Review
- Short-Term Dynamic Unloading of Bovine Tail Discs in Culture Partially Mitigates Induced Degeneration After One-Strike Trigger.JOR spine · 2025Article
- Multiaxial rotational loading compromises the transition zone of the intervertebral disc: Ex vivo study using next-generation bioreactors.Bioengineering & translational medicine · 2025Article
- Do we know more about the mechanobiology of the intervertebral disc in space than on Earth?JOR spine · 2025Article
- A repeated strike loading organ culture model for studying compression-associated chronic disc degeneration.Biomolecules & biomedicine · 2025Article
- Diverse and multifunctional roles for perlecan (JOR spine · 2024Review
- Thread-structural microneedles loaded with engineered exosomes for annulus fibrosus repair by regulating mitophagy recovery and extracellular matrix homeostasis.Bioactive materials · 2024Article
- Repetitive strikes loading organ culture model to investigate the biological and biomechanical responses of the intervertebral disc.JOR spine · 2024Article
- A new target for treating intervertebral disk degeneration: gut microbes.Frontiers in microbiology · 2024Review
- Kartogenin-enhanced dynamic hydrogel ameliorates intervertebral disc degeneration via restoration of local redox homeostasis.Journal of orthopaedic translation · 2023Article
- Chemonucleolysis combined with dynamic loading for inducing degeneration in bovine caudal intervertebral discs.Frontiers in bioengineering and biotechnology · 2023Article
- Intradiscal injection of human recombinant BMP-4 does not reverse intervertebral disc degeneration induced by nuclectomy in sheep.Journal of orthopaedic translation · 2022Article
- Toward the Next Generation of Spine Bioreactors: Validation of an Ex Vivo Intervertebral Disc Organ Model and Customized Specimen Holder for Multiaxial Loading.ACS biomaterials science & engineering · 2022Article
- Neoepitope fragments as biomarkers for different phenotypes of intervertebral disc degeneration.JOR spine · 2022Article
- Hyaluronic acid ameliorates intervertebral disc degenerationFrontiers in bioengineering and biotechnology · 2022Article
- New Progress in Basic Research of Macrophages in the Pathogenesis and Treatment of Low Back Pain.Frontiers in cell and developmental biology · 2022Review
- New Hope for Treating Intervertebral Disc Degeneration: Microsphere-Based Delivery System.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAcute trauma on intervertebral discs (IVDs) is thought to be one of the risk factors for IVD degeneration. The pathophysiology of IVD degeneration induced by single high impact mechanical injury is not very well understood. The aim of this study was using a post-traumatic IVD model in a whole organ culture system to analyze the biological and biomechanical consequences of the single high-impact loading event on the cultured IVDs.
methodsIsolated healthy bovine IVDs were loaded with a physiological loading protocol in the control group or with injurious loading (compression at 50% of IVD height) in the one strike loading (OSL) group. After another 1 day (short term) or 8 days (long term) of whole organ culture within a bioreactor, the samples were collected to analyze the cell viability, histological morphology and gene expression. The conditioned medium was collected daily to analyze the release of glycosaminoglycan (GAG) and nitric oxide (NO).
resultsThe OSL IVD injury group showed signs of early degeneration including reduction of dynamic compressive stiffness, annulus fibrosus (AF) fissures and extracellular matrix degradation. Compared to the control group, the OSL model group showed more severe cell death (P < 0.01) and higher GAG release in the culture medium (P < 0.05). The MMP and ADAMTS families were up-regulated in both nucleus pulposus (NP) and AF tissues from the OSL model group (P < 0.05). The OSL injury model induced a traumatic degenerative cascade in the whole organ cultured IVD.
conclusionsThe present study shows a single hyperphysiological mechanical compression applied to healthy bovine IVDs caused significant drop of cell viability, altered the mRNA expression in the IVD, and increased ECM degradation. The OSL IVD model could provide new insights into the mechanism of mechanical injury induced early IVD degeneration. THE TRANSLATIONAL POTENTIAL OF THIS ARTICLE: This model has a high potential for investigation of the degeneration mechanism in post-traumatic IVD disease, identification of novel biomarkers and therapeutic targets, as well as screening of treatment therapies.
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