Evidence map›Paper›PMID 33437445›Full record

ArticleEcology and evolution2021

A refined panel of 42 microsatellite loci to universally genotype catarrhine primates.

Franziska Trede, Niels Kil, James Stranks, Andrew Jesse Connell, Julia Fischer, Julia Ostner, Oliver Schülke, Dietmar Zinner, Christian Roos

Abstract read
In one paragraph

Article in Ecology and evolution, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Franziska TredeCognitive Ethology Laboratory German Primate Center Leibniz Institute for Primate Research Göttingen Germany.ORCID https://orcid.org/0000-0003-3690-1006
Niels KilPrimate Genetics Laboratory German Primate Center Leibniz Institute for Primate Research Göttingen Germany.ORCID https://orcid.org/0000-0002-8823-2599
James StranksPrimate Genetics Laboratory German Primate Center Leibniz Institute for Primate Research Göttingen Germany.ORCID https://orcid.org/0000-0002-5557-9051
Andrew Jesse ConnellDepartment of Microbiology Perelman School of Medicine University of Pennsylvania Philadelphia PA USA.ORCID https://orcid.org/0000-0002-9393-3074
Julia FischerCognitive Ethology Laboratory German Primate Center Leibniz Institute for Primate Research Göttingen Germany.ORCID https://orcid.org/0000-0002-5807-0074
Julia OstnerDepartment of Behavioral Ecology University of Göttingen Göttingen Germany.ORCID https://orcid.org/0000-0001-6871-9976
Oliver SchülkeDepartment of Behavioral Ecology University of Göttingen Göttingen Germany.ORCID https://orcid.org/0000-0003-0028-9425
Dietmar ZinnerCognitive Ethology Laboratory German Primate Center Leibniz Institute for Primate Research Göttingen Germany.ORCID https://orcid.org/0000-0003-3967-8014
Christian RoosPrimate Genetics Laboratory German Primate Center Leibniz Institute for Primate Research Göttingen Germany.ORCID https://orcid.org/0000-0003-0190-4266

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microsatellite genotyping is an important genetic method for a number of research questions in biology. Given that the traditional fragment length analysis using polyacrylamide gel or capillary electrophoresis has several drawbacks, microsatellite genotyping-by-sequencing (GBS) has arisen as a promising alternative. Although GBS mitigates many of the problems of fragment length analysis, issues with allelic dropout and null alleles often remain due to mismatches in primer binding sites and unnecessarily long PCR products. This is also true for GBS in catarrhine primates where cross-species amplification of loci (often human derived) is common.We therefore redesigned primers for 45 microsatellite loci based on 17 available catarrhine reference genomes. Next, we tested them in singleplex and different multiplex settings in a panel of species representing all major lineages of Catarrhini and further validated them in wild Guinea baboons (

Indexed as

apesgenotyping‐by‐sequencinghigh‐throughput sequencingOld World monkeyssimple tandem repeats

Identifiers

PMID33437445
PMCPMC7790618

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.