ArticleScientific reports2021
Microfluidic capillary networks are more sensitive than ektacytometry to the decline of red blood cell deformability induced by storage.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Microfluidic Chip Platforms for Red Blood Cell Storage Lesion Quality Control and Precision Transfusion.Research (Washington, D.C.) · 2026Review
- Evaluating anti-sickling therapies for sickle cell disease: a microfluidic assay for red blood cell-mediated microvascular occlusion under hypoxia.Lab on a chip · 2025Article
- Variability in the Deformability of Red Blood Cells: Application to Treating Premature Newborns with Blood Transfusion.International journal of molecular sciences · 2025Article
- New and emerging technologies for pretransfusion blood quality assessment: A state-of-the-art review.Transfusion · 2024Review
- Biomechanical Investigation of Red Cell Sedimentation Using Blood Shear Stress and Blood Flow Image in a Capillary Chip.Micromachines · 2023Article
- Big Data in Transfusion Medicine and Artificial Intelligence Analysis for Red Blood Cell Quality Control.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2023Review
- Deep ensemble learning enables highly accurate classification of stored red blood cell morphology.Scientific reports · 2023Article
- Biosensing of Haemorheological Properties Using Microblood Flow Manipulation and Quantification.Sensors (Basel, Switzerland) · 2022Article
- Cross-talk between red blood cells and plasma influences blood flow and omics phenotypes in severe COVID-19.eLife · 2022Article
- Red Blood Cell Sedimentation Index Using Shear Stress of Blood Flow in Microfluidic Channel.Biosensors · 2022Article
- Article
- Persistent red blood cells retain their ability to move in microcapillaries under high levels of oxidative stress.Communications biology · 2022Article
- Biochemical and Biophysical Properties of Red Blood Cells in Disease.Biomolecules · 2022Article
- OcclusionChip: A functional microcapillary occlusion assay complementary to ektacytometry for detection of small-fraction red blood cells with abnormal deformability.Frontiers in physiology · 2022Article
- Erysense, a Lab-on-a-Chip-Based Point-of-Care Device to Evaluate Red Blood Cell Flow Properties With Multiple Clinical Applications.Frontiers in physiology · 2022Article
- Rheological Abnormalities in Human Erythrocytes Subjected to Oxidative Inflammation.Frontiers in physiology · 2022Review
- Deformability of Stored Red Blood Cells.Frontiers in physiology · 2021Review
- Concurrent Assessment of Deformability and Adhesiveness of Sickle Red Blood Cells by Measuring Perfusion of an Adhesive Artificial Microvascular Network.Frontiers in physiology · 2021Article
- Effects of Coincubation With Crystalloids or Medications on Canine Packed Red Blood Cells: An In Vitro Evaluation.Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001)Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Ektacytometry has been the primary method for evaluating deformability of red blood cells (RBCs) in both research and clinical settings. This study was designed to test the hypothesis that the flow of RBCs through a network of microfluidic capillaries could provide a more sensitive assessment of the progressive impairment of RBC deformability during hypothermic storage than ektacytometry. RBC units (n = 9) were split in half, with one half stored under standard (normoxic) conditions and the other half stored hypoxically, for up to 6 weeks. RBC deformability was measured weekly using two microfluidic devices, an artificial microvascular network (AMVN) and a multiplexed microcapillary network (MMCN), and two commercially available ektacytometers (RheoScan-D and LORRCA). By week 6, the elongation indexes measured with RheoScan-D and LORRCA decreased by 5.8-7.1% (5.4-6.9% for hypoxic storage). Over the same storage duration, the AMVN perfusion rate declined by 27.5% (24.5% for hypoxic) and the MMCN perfusion rate declined by 49.0% (42.4% for hypoxic). Unlike ektacytometry, both AMVN and MMCN measurements showed statistically significant differences between the two conditions after 1 week of storage. RBC morphology deteriorated continuously with the fraction of irreversibly-damaged (spherical) cells increasing significantly faster for normoxic than for hypoxic storage. Consequently, the number of MMCN capillary plugging events and the time MMCN capillaries spent plugged was consistently lower for hypoxic than for normoxic storage. These data suggest that capillary networks are significantly more sensitive to both the overall storage-induced decline of RBC deformability, and to the differences between the two storage conditions, than ektacytometry.
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