ArticleScientific reports2021
Increasing AFM colloidal probe accuracy by optical tweezers.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Frequency-dependent cellular microrheology with pyramidal atomic force microscopy probes.Biophysical journal · 2026Article
- Traceable stiffness calibration of colloidal AFM probes for biomechanical measurements.Scientific reports · 2026Article
- Analysing Sources of Error in Total Internal Reflection Microscopy (TIRM) Experiments and Data Analysis.Polymers · 2023Article
- Surface characterization of an ultra-soft contact lens material using an atomic force microscopy nanoindentation method.Scientific reports · 2022Article
- Studies of probe tip materials by atomic force microscopy: a review.Beilstein journal of nanotechnology · 2022Review
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Authors and funding
5 authors.
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Abstract
A precise determination of the cantilever spring constant is the critical point of all colloidal probe experiments. Existing methods are based on approximations considering only cantilever geometry and do not take into account properties of any object or substance attached to the cantilever. Neglecting the influence of the colloidal sphere on the cantilever characteristics introduces significant uncertainty in a spring constant determination and affects all further considerations. In this work we propose a new method of spring constant calibration for 'colloidal probe' type cantilevers based on the direct measurement of force constant. The Optical Tweezers based calibration method will help to increase the accuracy and repeatability of the AFM colloidal probe experiments.
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