ArticleScientific reports2021
Optimal pulse length of insonification for Piezo1 activation and intracellular calcium response.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Impact of static and dynamic magneto-mechanical stimulation with vortex microdiscs on glioblastoma cells cultured on 2D substrates with physiological stiffness.Nanoscale advances · 2026Article
- Precise Regulation of Membrane Proteins: From Physical Technology to Biomolecular Strategy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Molecular and biophysical characterization of cultured DRG neurons in response to focused ultrasound.Scientific reports · 2026Article
- Diagnostic ultrasound-microbubble therapy promotes wound healing through miR-26b-5p-mediated GSK3β suppression.Scientific reports · 2025Article
- Enhancing cancer therapyLab on a chip · 2025Article
- Neuromodulation with Ultrasound: Hypotheses on the Directionality of Effects and Community Resource.medRxiv : the preprint server for health sciences · 2025Article
- TRPC6 is a mechanosensitive channel essential for ultrasound neuromodulation in the mammalian brain.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Sonogenetics in the Treatment of Chronic Diseases: A New Method for Cell Regulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Force versus Response: Methods for Activating and Characterizing Mechanosensitive Ion Channels and GPCRs.Advanced healthcare materials · 2024Review
- Effects of insonification on repairing the renal injury of diabetic nephropathy rats.BMJ open diabetes research & care · 2024Article
- Facilitatory effect of low-pulse repetition frequency ultrasound on release of extracellular vesicles from cultured myotubes.Journal of medical ultrasonics (2001) · 2024Article
- Subconductance states add complexity to Piezo1 gating model.Trends in biochemical sciences · 2024Article
- Transcranial focused ultrasound precise neuromodulation: a review of focal size regulation, treatment efficiency and mechanisms.Frontiers in neuroscience · 2024Review
- The principles and promising future of sonogenetics for precision medicine.Theranostics · 2024Review
- Linking the Mechanics of Chewing to Biology of the Junctional Epithelium.Journal of dental research · 2023Article
- Intercellular Calcium Waves and Permeability Change Induced by Vertically Deployed Surface Acoustic Waves in a Human Cerebral Microvascular Endothelial Cell Line (hCMEC/D3) Monolayer.Ultrasound in medicine & biology · 2023Article
- Genetically encoded mediators for sonogenetics and their applications in neuromodulation.Frontiers in cellular neuroscience · 2023Review
- First Series Using Ultrasonic Propulsion and Burst Wave Lithotripsy to Treat Ureteral Stones.The Journal of urology · 2022Article
- Acoustofluidic Stimulation of Functional Immune Cells in a Microreactor.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Focused ultrasound excites action potentials in mammalian peripheral neurons in part through the mechanically gated ion channel PIEZO2.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Ultrasound (US) neuromodulation, especially sonogenetics, has been demonstrated with potential applications in noninvasive and targeted treatment of various neurological disorders. Despite the growing interest, the mechanism for US neuromodulation remains elusive, and the optimal condition for eliciting a neural response with minimal adverse effect has not been identified. Here, we investigate the Piezo1 activation and intracellular calcium response elicited by acoustical streaming induced shear stress under various US exposure conditions. We find that Piezo1 activation and resultant intracellular calcium response depend critically on shear stress amplitude and pulse length of the stimulation. Under the same insonification acoustic energy, we further identify an optical pulse length that leads to maximum cell deformation, Piezo1 activation, and calcium response with minimal injury, confirmed by numerical modeling of Piezo1 channel gating dynamics. Our results provide insight into the mechanism of ultrasonic activation of Piezo1 and highlight the importance of optimizing US exposure conditions in sonogenetics applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.