Evidence map›Paper›PMID 33436634›Full record

ArticleScientific reports2021

Assembling mitogenome of Himalayan Black Bear (U. t. laniger) from low depth reads and its application in drawing phylogenetic inferences.

Amrita Bit, Mukesh Thakur, Sujeet Kumar Singh, Bheem Dutt Joshi, Vinay Kumar Singh, Lalit Kumar Sharma, Basudev Tripathy, Kailash Chandra

Abstract read
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Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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3 · Its place in the literature

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2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Amrita BitZoological Survey of India, New Alipore, Kolkata, West Bengal, 700053, India.
Mukesh ThakurZoological Survey of India, New Alipore, Kolkata, West Bengal, 700053, India. thamukesh@gmail.com.
Sujeet Kumar SinghZoological Survey of India, New Alipore, Kolkata, West Bengal, 700053, India.
Bheem Dutt JoshiZoological Survey of India, New Alipore, Kolkata, West Bengal, 700053, India.
Vinay Kumar SinghZoological Survey of India, New Alipore, Kolkata, West Bengal, 700053, India.
Lalit Kumar SharmaZoological Survey of India, New Alipore, Kolkata, West Bengal, 700053, India.
Basudev TripathyZoological Survey of India, New Alipore, Kolkata, West Bengal, 700053, India.
Kailash ChandraZoological Survey of India, New Alipore, Kolkata, West Bengal, 700053, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The complete mitogenome of Himalayan black bear (Ursus thibetanus laniger) from Indian Himalayan region was assembled following the modified approach of mitochondrial baiting and mapping using the next-generation sequencing reads. The complete mitogenome was of 16,556 bp long, consisted of 37 genes that contained 13 protein-coding genes, 22 tRNAs, 2 rRNAs and 1 control region. The complete base composition was 31.33% A, 15.24% G, 25.45%C, and 27.98%T and gene arrangement was similar to the other sub-species of Asiatic black bear. The relative synonymous codon usage analysis revealed the maximum abundance of Isoleucine, Tyrosine, Leucine and Threonine. The assembled mitogenome of U. t. laniger exhibited 99% similarity with the mitogenomes of Himalayan black bear available from Nepal and Tibetan Plateau-Himalaya region. The findings of the present study has proven low depth sequencing data, adequate and highly efficient in rapid recovering the mitochondrial genome by overcoming the conventional strategies of obtaining long-range PCR and subsequently drawing phylogenetic inferences.

Indexed as

Genome, MitochondrialPhylogenyAnimalsDNA, MitochondrialHigh-Throughput Nucleotide SequencingMitochondriaUrsidaeDNA, Mitochondrial

Identifiers

PMID33436634
PMCPMC7803731

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