ArticleNature communications2021
Capsid-like particles decorated with the SARS-CoV-2 receptor-binding domain elicit strong virus neutralization activity.
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Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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84citing papers in PubMed
7.3field-weighted citation impact, top 2% of its field
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Who cites it
84 citing papers in PubMed, 127 citations in OpenAlex.
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- Surface-bioengineering of bacteriophage AP205 and MS2 virus-like particles with novel Spytag003 for antigen conjugation.Journal, genetic engineering & biotechnology · 2026Article
- The inhibition of the Aβ-ASC interaction site suppresses β-amyloid aggregation and cytotoxicity.Frontiers in immunology · 2026Article
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- A novel nanoparticle vaccine, based on S1-CTD, elicits robust protective immune responses against porcine deltacoronavirus.Journal of virology · 2025Article
- Colloidal Crystallization of Virus-Like Particles with Polycations.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- SARS-CoV-2 RBD Scaffolded by AP205 or TIP60 Nanoparticles and Delivered as mRNA Elicits Robust Neutralizing Antibody Responses.Vaccines · 2025Article
- A modular mRNA vaccine platform encoding antigen-presenting capsid virus-like particles enhances the immunogenicity of the malaria antigen Pfs25.Nature nanotechnology · 2025Article
- A Modular Bacteriophage T4 Nanoparticle Platform Enables Rapid Design of Dual COVID-19-Flu Mucosal Vaccines.Small science · 2025Article
- Functional antibody responses to SARS-CoV-2 variants before and after booster vaccination among adults in Ghana.Experimental biology and medicine (Maywood, N.J.) · 2025Article
- Protein nanoparticle vaccines induce potent neutralizing antibody responses against MERS-CoV.Cell reports · 2024Article
- A self-adjuvanted VLPs-based Covid-19 vaccine proven versatile, safe, and highly protective.Scientific reports · 2024Article
- Broadly potent spike-specific human monoclonal antibodies inhibit SARS-CoV-2 Omicron sub-lineages.Communications biology · 2024Article
- Molecular Design of Encapsulin Protein Nanoparticles to Display Rotavirus Antigens for Enhancing Immunogenicity.Vaccines · 2024Article
- Bacteriophage T4 as a Protein-Based, Adjuvant- and Needle-Free, Mucosal Pandemic Vaccine Design Platform.Annual review of virology · 2024Review
- The role of PCSK9 in heart failure and other cardiovascular diseases-mechanisms of action beyond its effect on LDL cholesterol.Heart failure reviews · 2024Review
- Genetic Functionalization of Protein-Based Biomaterials via Protein Fusions.Biomacromolecules · 2024Review
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- Protection of K18-hACE2 Mice against SARS-CoV-2 Challenge by a Capsid Virus-like Particle-Based Vaccine.Vaccines · 2024Article
24 more citing papers are in PubMed but not listed here.
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Authors and funding
47 authors at 9 institutions in 6 countries.
Cyrielle FougerouxAdaptVac Aps, 2970, Hørsholm, Denmark.ORCID http://orcid.org/0000-0002-7566-8377
Louise GoksøyrAdaptVac Aps, 2970, Hørsholm, Denmark.ORCID http://orcid.org/0000-0003-4508-9857
Manja IdornDepartment of Biomedicine, Aarhus University, 8000, Aarhus, Denmark.ORCID http://orcid.org/0000-0002-6769-9165
Vladislav SorokaExpreS2ion Biotechnologies Aps, 2970, Hørsholm, Denmark.
Sebenzile K MyeniDepartment of Medical Microbiology, Leiden University Medical Center, ZA, Leiden, 2333, Netherlands.
Robert DagilCentre for Medical Parasitology at Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Disease, Copenhagen University Hospital, 2200, Copenhagen, Denmark.ORCID http://orcid.org/0000-0002-5594-0716
Christoph M JanitzekCentre for Medical Parasitology at Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Disease, Copenhagen University Hospital, 2200, Copenhagen, Denmark.
Max SøgaardExpreS2ion Biotechnologies Aps, 2970, Hørsholm, Denmark.
Kara-Lee AvesCentre for Medical Parasitology at Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Disease, Copenhagen University Hospital, 2200, Copenhagen, Denmark.ORCID http://orcid.org/0000-0002-0068-7733
Emma W HorstedCentre for Medical Parasitology at Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Disease, Copenhagen University Hospital, 2200, Copenhagen, Denmark.
Sayit Mahmut ErdoğanCentre for Medical Parasitology at Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Disease, Copenhagen University Hospital, 2200, Copenhagen, Denmark.ORCID http://orcid.org/0000-0001-7120-1609
Tobias GustavssonCentre for Medical Parasitology at Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Disease, Copenhagen University Hospital, 2200, Copenhagen, Denmark.
Jerzy DoroszExpreS2ion Biotechnologies Aps, 2970, Hørsholm, Denmark.
Stine ClemmensenExpreS2ion Biotechnologies Aps, 2970, Hørsholm, Denmark.
Laurits FredsgaardCentre for Medical Parasitology at Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Disease, Copenhagen University Hospital, 2200, Copenhagen, Denmark.ORCID http://orcid.org/0000-0003-3891-3414
Susan ThraneAdaptVac Aps, 2970, Hørsholm, Denmark.
Elena E Vidal-CalvoVAR2pharmaceuticals, 2200, Copenhagen, Denmark.ORCID http://orcid.org/0000-0001-9655-2106
Paul KhaliféCentre for Medical Parasitology at Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Disease, Copenhagen University Hospital, 2200, Copenhagen, Denmark.ORCID http://orcid.org/0000-0001-6160-4674
Thomas M HulenCentre for Medical Parasitology at Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Disease, Copenhagen University Hospital, 2200, Copenhagen, Denmark.ORCID http://orcid.org/0000-0003-2475-6526
Swati ChoudharyCentre for Medical Parasitology at Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Disease, Copenhagen University Hospital, 2200, Copenhagen, Denmark.
Michael TheisenCentre for Medical Parasitology at Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Disease, Copenhagen University Hospital, 2200, Copenhagen, Denmark.
Susheel K SinghCentre for Medical Parasitology at Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Disease, Copenhagen University Hospital, 2200, Copenhagen, Denmark.
Asier Garcia-SenosiainCentre for Medical Parasitology at Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Disease, Copenhagen University Hospital, 2200, Copenhagen, Denmark.
Linda Van OostenDepartment of Plant Sciences, Laboratory of Virology, 6700AA, Wageningen, Netherlands.
Gorben PijlmanDepartment of Plant Sciences, Laboratory of Virology, 6700AA, Wageningen, Netherlands.ORCID http://orcid.org/0000-0001-9301-0408
Bettina HierzbergerExpreS2ion Biotechnologies Aps, 2970, Hørsholm, Denmark.ORCID http://orcid.org/0000-0001-5145-5265
Tanja DomeyerExpreS2ion Biotechnologies Aps, 2970, Hørsholm, Denmark.ORCID http://orcid.org/0000-0002-3908-9546
Blanka W NalewajekExpreS2ion Biotechnologies Aps, 2970, Hørsholm, Denmark.
Anette StrøbækExpreS2ion Biotechnologies Aps, 2970, Hørsholm, Denmark.ORCID http://orcid.org/0000-0001-9713-1119
Magdalena SkrzypczakExpreS2ion Biotechnologies Aps, 2970, Hørsholm, Denmark.ORCID http://orcid.org/0000-0001-6059-5059
Laura F AnderssonExpreS2ion Biotechnologies Aps, 2970, Hørsholm, Denmark.
Søren BuusDepartment of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200, Copenhagen, Danmark.ORCID http://orcid.org/0000-0001-8363-1999
Anette Stryhn BuusDepartment of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200, Copenhagen, Danmark.
Jan Pravsgaard ChristensenDepartment of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200, Copenhagen, Danmark.ORCID http://orcid.org/0000-0002-4299-9479
Tim J DaleboutDepartment of Medical Microbiology, Leiden University Medical Center, ZA, Leiden, 2333, Netherlands.
Kasper IversenDepartment of Cardiology, Herlev Hospital, 2730, Herlev, Denmark.
Lene H HarritshøjDepartment of Clinical Immunology, Copenhagen University Hospital, 2100, Copenhagen, Denmark.
Benjamin MordmüllerUniversitätsklinikum Tübingen, Institut für Tropenmedizin, 72074, Tübingen, Germany.ORCID http://orcid.org/0000-0001-9101-2768
Henrik UllumDepartment of Cardiology, Herlev Hospital, 2730, Herlev, Denmark.
Line S ReinertDepartment of Biomedicine, Aarhus University, 8000, Aarhus, Denmark.ORCID http://orcid.org/0000-0002-8317-0886
Willem Adriaan de JonghAdaptVac Aps, 2970, Hørsholm, Denmark.
Marjolein KikkertDepartment of Medical Microbiology, Leiden University Medical Center, ZA, Leiden, 2333, Netherlands.ORCID http://orcid.org/0000-0002-5779-7386
Søren R PaludanDepartment of Biomedicine, Aarhus University, 8000, Aarhus, Denmark.ORCID http://orcid.org/0000-0001-9180-4060
Thor G TheanderCentre for Medical Parasitology at Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Disease, Copenhagen University Hospital, 2200, Copenhagen, Denmark.
Morten A NielsenCentre for Medical Parasitology at Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Disease, Copenhagen University Hospital, 2200, Copenhagen, Denmark. mortenn@sund.ku.dk.ORCID http://orcid.org/0000-0003-2668-4992
Ali SalantiCentre for Medical Parasitology at Department for Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Disease, Copenhagen University Hospital, 2200, Copenhagen, Denmark.
Adam F SanderAdaptVac Aps, 2970, Hørsholm, Denmark. asander@sund.ku.dk.ORCID http://orcid.org/0000-0002-8782-7830
University of Copenhagen · DKExpreS2ion Biotechnologies (Denmark) · DKAarhus University · DKLeiden University Medical Center · NLStatens Serum Institut · DKGraduate School Experimental Plant Sciences · NLHerlev Hospital · DKCopenhagen University Hospital · DKUniversitätsklinikum Tübingen · DE
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rapid development of a SARS-CoV-2 vaccine is a global priority. Here, we develop two capsid-like particle (CLP)-based vaccines displaying the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. RBD antigens are displayed on AP205 CLPs through a split-protein Tag/Catcher, ensuring unidirectional and high-density display of RBD. Both soluble recombinant RBD and RBD displayed on CLPs bind the ACE2 receptor with nanomolar affinity. Mice are vaccinated with soluble RBD or CLP-displayed RBD, formulated in Squalene-Water-Emulsion. The RBD-CLP vaccines induce higher levels of serum anti-spike antibodies than the soluble RBD vaccines. Remarkably, one injection with our lead RBD-CLP vaccine in mice elicits virus neutralization antibody titers comparable to those found in patients that had recovered from COVID-19. Following booster vaccinations, the virus neutralization titers exceed those measured after natural infection, at serum dilutions above 1:10,000. Thus, the RBD-CLP vaccine is a highly promising candidate for preventing COVID-19.
Indexed as
Angiotensin-Converting Enzyme 2AnimalsAntibodies, NeutralizingAntibodies, ViralCapsidCOVID-19COVID-19 VaccinesFemaleHumansImmunogenicity, VaccineKineticsMiceMice, Inbred BALB CProtein BindingRecombinant ProteinsSARS-CoV-2ACE2 protein, humanAngiotensin-Converting Enzyme 2Antibodies, NeutralizingAntibodies, ViralCOVID-19 VaccinesRecombinant ProteinsSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2
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